I want to know the chemical structure and molecular formula of Acid Blue dye CI 221
Is it possible to get two isoenzymes with same molecular weight? As, after doing anion exchange chromatography on a DEAE-cellulose column, two peaks of the enzyme activity were resolved by the...
07 August 2019 2,852 4 View
Could you help in prevention of sodium phytate from precipitation in the enzymatic reaction mixture for phytase assay at pH 5.5 using sodium acetate buffer? Also is it possible to use citrate...
05 June 2018 681 2 View
Is it possible to acknowledge the language editing person in the acknowledgement part in the manuscript?
06 July 2016 1,734 3 View
If I want to say one unit of enzyme is the amount of enzyme that able to produce one micro mole of the product, How I can calculate the micro moles produced from the enzymatic reaction? Thanks
10 November 2015 2,901 5 View
How do I know that my column is regenerated effectively after usage?
31 December 2012 4,678 4 View
Km, Vmax
09 October 2012 8,341 8 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I have multiple small samples of grass pollen with anthers. I want to find a method to identify concentrations of proteins, lipids, and other various elements for these samples. any suggestions?
28 July 2024 5,331 1 View
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
28 July 2024 3,559 0 View
I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
23 July 2024 3,891 0 View
I need to know the approximate size of my target protein for Negative-stain EM. I have an alpha-fold predicted structure of the protein. I do not see any turtorial on internet for estimating the...
21 July 2024 5,732 3 View