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02 March 2021 3,060 3 View

Does anyone know of tested survey questions or value statements to evaluate consumer ethics for food and agriculture?

I'm looking for tested survey questions or value statement that address topics of food and agricultural ethics and consumer values for food purchasing... is anyone aware of an existing study that...

01 July 2020 301 2 View

What is the best DNA extraction protocol to use for extremely oligotrophic soils?

We are trying to extract DNA from soil samples, these samples are collected from the Namib Desert, the site is about 20 Kilometers from the Atlantic Ocean. The soil has a high salt concentration...

26 October 2019 9,423 3 View

Is there a good replacement kit for he Ribo-Zero Gold rRNA Removal Kit (Epidemiology)?

Since Illumina announced that they will stop selling the Ribo-Zero Epidemiology kit, I have been looking for a good replacement kit. It will be a problem for people planning to use this kit,...

16 October 2018 432 5 View

Can anyone suggest a non-parametric version of a within-between repeated measures ANOVA?

06 August 2017 1,937 3 View

Fslchfiletype for SPM usage: orientation?

10 August 2016 3,004 2 View

How come my cresol purple solution (cf SOP 6b, Dickson) has no absorbance peak at 578 nm?

19 July 2016 9,230 6 View

Why does Tris has low-conductivity?

25 January 2016 2,807 1 View

Is there a workflow for analyzing microRNA data in Galaxy?

01 December 2014 1,079 6 View

Best method for bacterial DNA extraction from intestinal biopsies (small sample size)?

I have recently used the DNEasy tissue kit to extract DNA from intestinal biopsies with the goal of amplifying the 16SrRNA gene for bacteria for sequencing. I know there are many kits on the...

30 August 2012 7,436 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View