Hello everyone,

regarding bisulphate treatment, if the sample contains degraded RNA could this affect the efficiency of DNA conversion ?! and what to do to overcome this problem. as you can see from the gel pic for DNA samples below there is degraded RNA. and also the ratio 260/280 are higher than it supposes to be!

4ul from the samples were run on 0.8%agaroe gel

sample 1 conc.=153.1ng/ul with ratios 260/280=1.92 ,260/230=1.92

sample 2 conc.=187.8ng/ul with ratios 260/280=2.01 ,260/230=2.14

sample 3 conc.=840.8 ng/ul with ratios 260/280=2.05,260/230=2.09

Thank you in advance