I would like to know a good centrifugation protocol and which gel is better (12%, 15%, 18%..)
I have performed a western blot with the same samples, but I had problems in my antibodies, so I did once again. However, after the transfer to membrane (ECL nitrocelulose, amersham) only the...
07 August 2014 6,684 10 View
I need to homogenize tissue for blotting. However I have a small amount of tissue, it is mice's Accumbens. I tried tissue homogenizer, but it is too big for my tissue. Using pipete it is not...
31 December 2012 7,872 4 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
15 July 2024 2,055 3 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...
14 July 2024 9,324 2 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,165 1 View
Hello! I am planning a western blot experiment, and I am looking for GAPDH antibodies to order. In the past, I have experienced a lot of hit-or-miss when ordering antibodies based on just the...
07 July 2024 1,685 0 View
eliminoar articulos que son de tu autoria
05 July 2024 2,107 1 View
I have this problem with my western blot for the ABCG2 antibody on nitrocellulose membrane. Help please
02 July 2024 8,715 2 View