Douglas Engelke

3 Questions 9 Answers 0 Followers

Questions related from Douglas Engelke

Western Blot problem in transfer: Standard Transferred and protein not! Does anyone know why?

I have performed a western blot with the same samples, but I had problems in my antibodies, so I did once again. However, after the transfer to membrane (ECL nitrocelulose, amersham) only the...

08 August 2014 6,657 10 View

Could someone send me a good protocol for Histone H3-K9/K14 extraction and Western Blot analysis?

I would like to know a good centrifugation protocol and which gel is better (12%, 15%, 18%..)

02 February 2013 1,470 0 View

How can I homogenize small amout of tissue for Western Blotting?

I need to homogenize tissue for blotting. However I have a small amount of tissue, it is mice's Accumbens. I tried tissue homogenizer, but it is too big for my tissue. Using pipete it is not...

01 January 2013 7,850 4 View