I'm working in a project related to Ca2+ signalling and I've to detect protein expression of M1/M3 Acetylcholine Receptors and SERCA1/2/3. Could someone recommend good antibodies for detecting these genes through WB/IHC/ICC?
Thank you very much.
ChAT Monoclonal antibody (Boehringer, Mannheim, Germany) or any company produce this antibody.
Also you can used the Ach histochemistry ( Karnovsky and roots, 1964) modified by( Kujut etal 1993)
Kujat R1, Rose C, Wrobel KH. 1993 The innervation of the bovine ductus deferens: comparison of a modified acetylcholinesterase-reaction with immunoreactivities of cholinacetyltransferase and panneuronal markers. Histochemistry. 1993 Mar;99(3):231-9.
If there are no more professional recommendations, I recommend these antibodies on the creative Diagnostics website. You can have a try. Good Luck!
AChR: https://www.creative-diagnostics.com/symbolsearch_AChR.htm
SERCA1 : https://www.creative-diagnostics.com/symbolsearch_ATP2A1.htm
SERCA2: https://www.creative-diagnostics.com/symbolsearch_ATP2A2.htm
SERCA3: https://www.creative-diagnostics.com/symbolsearch_ATP2A3.htm
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