Nguyen Toan

3 Questions 2 Answers 0 Followers

Questions related from Nguyen Toan

Could someone recommend good antibodies for detecting M1, M3 AchR and SERCA1/2/3 in WB and IHC?

I'm working in a project related to Ca2+ signalling and I've to detect protein expression of M1/M3 Acetylcholine Receptors and SERCA1/2/3. Could someone recommend good antibodies for detecting...

09 September 2019 4,202 2 View

Too many unspecific bands when I run Western Blot, what should I do?

I ran Western Blot to detect Egr-1 protein from MC3T3 cell lines, but my blot has too much unspecific bands so I can't detect protein I want. Here is my work flow, the reagents I used, and my...

04 April 2019 5,711 7 View

Is anyone familiar with extraction of DNA from blood by Phenol - Chloroform - Isoamyl Alcohol solution?

I'm using Phenol - Chloroform - Isoamyl Alcohol solution to extract DNA from blood. But the OD result show me the DNA concentration is too high - approximate 300ng/ul. (in the attachment)Then I...

05 May 2015 646 6 View