After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Why do irreversible inhibitors only affect Vmax and not Km?

So I've been wondering why irreversible inhibitors only affect maximal velocity and not Km? Is there a...

19 January 2021 2,509 1 View

In the Venusian atmosphere why doesn't Nitrogen which is much lighter Carbon Dioxide float to the upper regions?

Carbon dioxide on Earth weighs 44 g/mol and has a density of around 1.98 kg/m3, about 1.53 times that of air. Nitrogen gas weighs 28 g/mol and has a density of 1.2506 g/L. During limnic eruptions...

15 January 2021 8,733 4 View

What type of cellular structures are these likely to be?

I have immunohistochemistry slides of human subcutaneous fat biopsy, with skin attached. Most of the structures are self-evident - skin, adipocytes, microvessels in transverse section (revealed by...

03 December 2020 4,238 7 View

Mata-analysis question: How to deal with multiple outcome scales used within individual studies?

Dear all, thanks for the help in advance. I am performing a met-analysis. Most of the studies included report the same outcome using multiple scales e.g. MMSE or CDRS. What is the best way of...

30 October 2020 8,629 5 View

Glucose dehydrogenase interference with glucokinase assay?

Hello all, I'm currently working with undergraduate student-researchers (there are no graduate students at my university) to purify and characterize the drug targetability of glucokinase from pig...

25 October 2020 3,553 7 View

What is the minimum number of timepoints for markov models?

For those who frequently work in markov models, I am interested in knowing the minimum number of time points/observations to get stable estimates of parameters. I have some follow ups, too:...

24 September 2020 9,659 3 View

How best to address statistical differences when there is no difference in the independent variable?

If an experiment includes, say, two groups and Condition 1 is identical between groups but Condition 2 differs between groups, what is the best course of action if one finds a significant...

17 September 2020 9,731 7 View

Can you re-use ethidium bromide post-stain solution more than once, or does ethidium bromide need to be added fresh each time?

When post-staining agarose gels in an ethidium bromide / water bath for 10 minutes, my labmate says she can re-use the post-stain solution 2-3 times before she has to add more ethidium bromide (at...

04 September 2020 680 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

How to extract a single stranded DNA from a double stranded DNA sample isolated from stem borer(rice affecting insect) ?

How to extract a single-stranded DNA from a double-stranded DNA sample isolated from stem borer(rice affecting insect)? As I need to amplify ss Dna using a COX-1 marker having primer sequence as...

22 January 2021 3,424 3 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

Detection of insect eggs in stored and processed products?

Currently, in order to identify insect eggs in stored or processed products (such as contaminated flour or wafer samples), staining was performed based on the method mentioned in the below...

15 January 2021 6,362 5 View