Issues with my microglia primary culture?

Hi everyone, I'm isolating microglia from P1-2 mice and seeding microglia cells from 3 pups brains/T-25 flask. After seeding, I let this astrocytes-microglia mix culture grow for approx 2-3 weeks,...

06 October 2020 3,916 3 View

Do you have any suggestion for a easy and cheap experiment to quantify Cytokines?

I need to test the stimulation production of TNF alpha in the RAW cell line by LPS. I am looking for an easy and cheap experiment to quantify the serial dilution stimulation in this macrophage...

27 September 2020 5,366 6 View

Good antibodies for D1 and D2 striatal medium spiny neurons?

Hello! I am looking for good antibodies that help in clearly distinguish between D1- and D2-type medium spiny neurons (MSNs) in the striatum, by immunohistochemistry and immunofluorescent...

31 May 2020 5,009 3 View

Good antibodies for rat M1 and M2 microglia?

Hello! Do you know some good antibodies, suitable for IFL, for differently staining the M2 and M2 microglia phenotypes in rat tissue? I am searching for antibodies specific for RAT because all the...

27 October 2019 7,260 3 View

Anyone can help me about grayanotoxin analytical standards for LC-MS/MS?

I need to buy analytical standards for grayanotoxin I, II and III. I only find the type I. Roberta Merlanti

15 May 2019 2,628 4 View

Can anyone recommend a good paper that describes a work on separation of both tannins and saponins?

I'm having a hard time to work on these metabolites.

13 February 2015 514 3 View

Is THPS toxic towards methanogenic bacteria? In case it is, is there a "selective (more or less!) biocide against sulphate reducing bacteria?

Tetrakis Hydroxymethyl Phosphonium Sulphate and methanogenic bacteria

21 September 2014 8,375 2 View

Any alternatives to 3MA solubilization?

I have to use 3-MA in my cell culture at high concentration. II know that 3-MA is not very soluble in water, so the solution would be solubilize 3-MA in DMSO, but I know that DMSO generally...

08 June 2014 1,658 3 View

What types of transfection reagents do people use to transfect neurons, IPS derived neurons, astrocytes and neural stem cells?

Do more people use cDNA, siRNA, mRNA or miRNA? Would love to hear what people are using?

29 January 2014 1,147 7 View

What types of transfection reagents are you using to transfect neurons, astrocytes, IPS neurons?

cDNA, mRNA, siRNA, miRNA

27 January 2014 4,591 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View