Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

Picrosirius red staining without picric acid?

I would like to perform picrosirius red staining but we cannot use picric acid anymore. Do anyone have already test other acid like citric acid instead? Thanks a lot

08 September 2020 2,794 2 View

Dopamine transporter antibody?

I am looking for an antibody against dopamine transporter suitable for WB. I have tried a couple of antibodies but I obtained poor results. Can anyone help me?

18 May 2020 1,536 2 View

Experience with siRNA transfection of neural stem cells?

Does anyone have experience in gene silencing through siRNA transfection specifically in neural stem cells (ReN cells)? I know these are hard to transfect cells, and with other methods I normally...

29 March 2020 4,210 0 View

Can anybody help me with Boyden chamber invasion assay for HCT-116 cell lines?

I have a problems with Boyden chamber assay for HCT-116 cell lines, I did not see any stained cell in the membrane. I have had a success with other cell line, A549. Does anyone have any...

23 March 2020 7,119 5 View

How to make cell distribute evenly in 12-well plate?

I worked with cell culture 1 year ago, nearly I have a problem when I seeding in 12-well plate, more cells settles in the middle of well. I am trying with different 'shaking method'. I hope to get...

19 March 2020 9,999 3 View

Can anyone provide the Scoring algorithm for the UK SF-12 Version 1?

24 February 2020 1,653 2 View

How can I calculate the total average (or median) if I have 10 samples averages with different sizes?

I want to do some bibliography research and I have a table with the average age of 10 samples. Each sample has different size. Also I don't have the SDfor every dataset info.

07 January 2020 1,852 3 View

Can anyone recommend any strong papers concerning the prevalence of PTSD and/or CPTSD in paramedics?

Dear all, I am in the early stages of undertaking research surrounding PTSD/CPTSD and the prevalence of these conditions in paramedics. Can anyone point me towards some recent research in this...

29 October 2019 8,117 1 View

How long after my last allergen challenge can I measure Mouse Mast cell protease-1 (mMCP-1)?

Hi: I have lung tissues I can homogenize to measure different cytokines. I am also interested in measuring some Mast cells proteases if possible(specifically mMCP-1). The problem is, lung tissues...

15 October 2019 9,291 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View

For PC-3 cell line. How long before irradiation do I have to synchronize my cells to G0/G1?

I am trying to do a clonogenic assay for PC-3 after irradiation. I have to synchronize my cells by serum starvation but there's a optimal time I have to do that before irradiation. been looking in...

09 February 2021 9,480 1 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View