I have tried to analyze microcystins by HPLC-PDA, but I couldn't get any peak which have absorbed at 238 nm. I don't know what's problem here.

Details about my analysis processing: 

- I have used the protocol described by Meriluoto and Cood (2005) and the protocol from Lawton et al. 1994.

- Standards from Enzo Sciences: MC-LR and MC-RR

- HPLC-PDA equipment

- Column: Luna 5u C18(2) 100A Phenomenex (250 * 4.6 mm)

Could someone give me advices about this problem?

Thank you very much!

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