I have tried to analyze microcystins by HPLC-PDA, but I couldn't get any peak which have absorbed at 238 nm. I don't know what's problem here.
Details about my analysis processing:
- I have used the protocol described by Meriluoto and Cood (2005) and the protocol from Lawton et al. 1994.
- Standards from Enzo Sciences: MC-LR and MC-RR
- HPLC-PDA equipment
- Column: Luna 5u C18(2) 100A Phenomenex (250 * 4.6 mm)
Could someone give me advices about this problem?
Thank you very much!