16 May 2018 19 7K Report

I am completing an article based on my empirical study and was wondering where to submit it.

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Is it Ok to decline to write a chapter for a IntechOpen to the lack of experience?

I have been asked by IntechOpen to write the chapter for a book they are going to publish, but as I don't have enough experience in the area they proposed me to write I declined their offer. Now...

24 February 2021 2,802 4 View

How to inerpret whether PCR went OK?

Dear friends! I am back to work after 3 years career break. Now performing a PCR, feeling like a newbie... Please, help to understand whether RT-PCR reaction "worked" or not. Which results should...

14 February 2021 3,129 8 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

EMOJI-POLL-3 (Emoji-Association): Can you join the survey?

https://docs.google.com/forms/d/e/1FAIpQLSfaaCWZZqMIhoz5yj_5dxggvb6-cISx3GUHssYUWZWKbpx3UA/viewform Dear colleagues! We ask you to participate in a series of surveys related to the scientific...

26 January 2021 5,969 3 View

The same lC-MS for Forensic Toxicological and Chemical expertises?

Dear Colleagues! How correct and appropriate is it to carry out a forensic toxicological examination of drugs and psychotropic substances in biological objects (in blood, urine, hair) and a...

24 January 2021 4,489 2 View

GC-Headspace (HS-GC) chromatography for Forensic toxicological and Food expertises?

Dear Colleagues! How correct and appropriate is it to conduct forensic toxicological and food examination of volatile substances (ethanol, methanol, propanol, ethyl glycol, etc.) in blood and...

24 January 2021 5,645 2 View

How to choose mouse cell line to work on?

Hey, I am looking to start working on mouse cell line for some genomics experiments. I have the following requirements: 1) Normal chromosomes composition. 2) Possible to transfect. 3) Possible to...

03 January 2021 4,387 2 View

How to describe the method described below?

I do research on the relationships between universities and local governments by asking the research question (how) can universities contribute to the design (and implementation) of public...

29 December 2020 4,522 12 View

How can I change the media for THP-1 cells without dislodging them from the surface of my well plate?

I am working with THP-1 cells at the moment and am having trouble with maintaining a high confluence once I seed them on a 24-well plate. I usually try to seed around 100,000 cells per well and...

29 December 2020 1,567 3 View

What glass vials do you use for small volumes (400ul-2000ul) that fit in the centrifuge?

I am planning some NMR experiments that require glass vials (due to chloroform). I am looking to find glass vials that would take 400-2000ul volumes, fit in the centrifuge and allow me to separate...

01 December 2020 3,259 2 View

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