Could somebody recommend me a protocol for bacteria DNA extraction from blood?

More Talia Marroquin's questions See All

How to calculate labeling efficiency for TMT10plex peptides?

Hi there, I used thermo's TMT 10plex kit to label peptides and pool them together for LCMS/MS analysis. I am analyzing my data in Proteome Discoverer 2.3 and wondering how to quantify the number...

19 January 2024 2,127 2 View

How to resolve warnings with Spectrum Files RC and Protein FDR Validator in Proteome Discoverer?

I am using Proteome Discoverer 2.3 to analyze TMT-labeled and phospho-enriched samples, but I am not getting any protein matches. I know there are relatively little peptides in these samples since...

17 January 2024 6,270 0 View

How To Improve factor loadings in CFA?

I am a PhD scholar. When importing the rotated component matrix in Amos 23, I'm having trouble. My factor loads while conducting CFA have different values than the loadings I get in SPSS after...

22 May 2023 6,437 6 View

Submission of article ?

I recently submitted an article in a journal titled International Journal of Disaster Resilience in the Built Environment but I was unaware of the submission date of abstract was Nov-25-2022 and I...

12 January 2023 1,131 3 View

How much time it takes for Prabandhan Journal to give the first desk decision?

I have sent my paper to Indian journal of management Prabadhan on 31st august 2022 and till now I havent got any response re;lated to that. How much time it takes for the first desk decision.

28 September 2022 4,456 2 View

How much plagirism similarity is allowed in writing research papers?

I am writing a paper and I am getting plagirism around 14 percent in my main varaibles. eg one of my varaibles is green human resource management (GHRM) and i am getting similarity mostly in this...

30 August 2022 3,681 8 View

Why my odds ratios are contradicting the percentage of incidence?

I am comparing two surgical approaches and the odds ratios favor A over B but when I calculate the percentage of incidence of complications the outcome favors B over A. Is this possible? under...

29 August 2021 9,576 11 View

Nonparametric alternative for planned comparison test?

Hi all! In a previous analysis, I've conducted a one-way anova with planned comparisons. As I've been advised to conduct some nonparametric tests instead, I'm looking for your help. I used the...

07 July 2021 5,382 4 View

How can I obtain a bacteria pellet from whole blood, or from plasma by centrifugation?

I have samples in 4ml vacutainer tubes Thanks

22 June 2021 9,757 5 View

COVID-19 / SARS-COV-2 and children, still it silent borne?

For discussion: Please read the following article, published last weekend, to discuss these points: - Children develop symptoms within the studied sample. - After discharged children which...

28 March 2020 6,729 6 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

Abdullah Ghawanmeh

Dear Talia,

Please check this link

https://www.thermofisher.com/jo/en/home/references/protocols/nucleic-acid-purification-and-analysis/dna-extraction-protocols/dna-extraction-from-blood.html

Good Luck

Talia Marroquin

Thank you

Abhijeet Singh

By the way bacteria is already a plural. Singular would be bacterium.

thank you