I think the proteins was trapped in column but i do not know is my idea true or not.
Protein trapping may be because of improper column washing or if your sample is a crude extract.?
i need to polymerize the isopropylmethacrylamide monomers to get a polymer with LCST around 37C. i read in some articles which said it depends on polymer molecular weight.
04 May 2016 3,906 4 View
i need a cancer cell line can express P-glycoproteins and also be susceptible to Doxorubicin.
03 April 2016 8,731 4 View
if we want to compare this polymer versus polyvinylpyrrolidone, which one is better to use as a thermosensitive polymer capable to transport a hydrophobic drug to a cell?
01 February 2016 2,767 3 View
i have some data about a recombinant enzyme and i want to calculate my enzyme unite. i have used the ellman method for enzyme activity test. ODfirst minite= 0.35 ODseconde minite=0.8 reaction...
04 May 2015 7,045 2 View
I have a home made procainamide sepharose 4B column that completely filled by contaminant proteins. Can I use of EDTA as cleaning agent? Is it possible the EDTA destroy the procainamide?
02 March 2015 1,013 5 View
I am working on the interaction of silver nanoparticle in different size with acetylcholinestrase. i saw the blue shift in UV-Vis peak for treated nanoparticle with protein than blank one.
11 December 2014 2,556 7 View
I treated the silver nanoparticle with acetylcholinestrase. data showed after 3 hours the maximum absorbance peak for nanoparticle-protein increased than blank one. in blank sample there is just...
11 December 2014 1,394 9 View
We have a protein in the buffer that contain Histidine amino acids.
07 August 2014 5,982 1 View
There are some references showing that we can use cellophane as a semipermeable membrane and electrophoresis.
03 April 2014 8,905 5 View
Biotic stress such as viral infection.
02 March 2014 1,625 7 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance
04 August 2024 9,492 0 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View