I think the proteins was trapped in column but i do not know is my idea true or not.
Protein trapping may be because of improper column washing or if your sample is a crude extract.?
i need to polymerize the isopropylmethacrylamide monomers to get a polymer with LCST around 37C. i read in some articles which said it depends on polymer molecular weight.
04 May 2016 3,811 4 View
i need a cancer cell line can express P-glycoproteins and also be susceptible to Doxorubicin.
03 April 2016 8,559 4 View
if we want to compare this polymer versus polyvinylpyrrolidone, which one is better to use as a thermosensitive polymer capable to transport a hydrophobic drug to a cell?
01 February 2016 2,639 3 View
i have some data about a recombinant enzyme and i want to calculate my enzyme unite. i have used the ellman method for enzyme activity test. ODfirst minite= 0.35 ODseconde minite=0.8 reaction...
04 May 2015 6,858 2 View
I have a home made procainamide sepharose 4B column that completely filled by contaminant proteins. Can I use of EDTA as cleaning agent? Is it possible the EDTA destroy the procainamide?
02 March 2015 903 5 View
I am working on the interaction of silver nanoparticle in different size with acetylcholinestrase. i saw the blue shift in UV-Vis peak for treated nanoparticle with protein than blank one.
11 December 2014 2,444 7 View
I treated the silver nanoparticle with acetylcholinestrase. data showed after 3 hours the maximum absorbance peak for nanoparticle-protein increased than blank one. in blank sample there is just...
11 December 2014 1,262 9 View
07 August 2014 5,879 1 View
There are some references showing that we can use cellophane as a semipermeable membrane and electrophoresis.
03 April 2014 8,788 5 View
Biotic stress such as viral infection.
02 March 2014 1,516 7 View
03 March 2021 8,272 1 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
02 March 2021 3,060 3 View
(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...
02 March 2021 3,309 2 View
What do you know about biotechnology art?
02 March 2021 1,653 3 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...
01 March 2021 9,701 5 View