How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

How to define least effective concentration (LEC) and maximum safe concentration (MSC) of drugs?

In drug delivery, for efficacy, the drug concentration needs to be within LEC and MSC. How can we define that concentration?

25 November 2020 6,478 3 View

Perception on business failure prediction models using the annual report?

27 July 2020 1,141 2 View

Any suggestions for tools to analysis, plotting and tabulating genomic data of bacteria genomes?

Hello, In order to analyse NGS results, I looking for some software or tool to tabulate the entire genome of a bacteria. Thanks in advance

27 July 2020 9,826 5 View

Dissertation Research: Business failure prediction. Could you help me with a short survey please?

I'm conducting a research study titled 'Perception on the business failure prediction using the annual report' to receive the degree of M.Sc. Accounting and Finance by Griffith College in Dublin,...

21 July 2020 8,761 3 View

Why does a small peptide precipitates in contact with albumin?

06 January 2020 8,673 5 View

Plotting my ordinal regressions results: which is the best graphic? Is it possible to use SPSS or Excel to plot this results?

I have many ordinal regressions with observed behavioural categories as VD (ordinal categories of 4 levels) and emotional state as VI (2 ordinal categories of 2 and 3 levels). I am using...

20 October 2018 5,243 3 View

What are the reasons for cell viability of more than 100% for DMSO control vehicle in MTT assay?

I performed MTT assay for assessing the cytotoxicity of curcumin (0-50uM) on LPS-stimulated THP-1 macrophages, and used DMSO as control vehicle. While calculating the cell viability% for DMSO, I...

26 June 2018 8,010 6 View

No title

As a control in my MAPK western blots I routinely use the anti-Fus3 antibody from Santa Cruz (SC-6772; YN-19SC), but recently learned that it has been discontinued from the catalogue. The same is...

07 February 2017 2,807 3 View

How can I retrieve lat and lon values of a Geotiff image?

Hi everybody, I try to extract values (lat and lon) of a Geotiff image that contains chlorophyll-a values, I need this information for compare with a MODIS values in MATLAB or another sofware.

08 September 2016 8,972 11 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View

How to find orthotropic material properties?

I am doing an impact analysis of bullet on a plate. But for that simulation, I require orthotropic properties of materials like PPS and PEEK which are Unidirectional type. How to find them?...

16 February 2021 8,775 2 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View

How can I multiply values with standard distribution and with triangular distribution?

I am calculating emissions considering the uncertainty of the data to do Monte Carlo simulation. Some variables such as yields have normal, lognormal or even uniform distributions. These must be...

03 February 2021 6,318 5 View

After cutting bands having low molecular weight peptides obtained from SDS-PAGE,is it possible to elute these peptide(s) without digesting in trypsin?

Basically, I want to get the sequence of peptides. I assume, digesting these peptides from each band may give me a different sequence than the original through LC-MS analysis. If digestion doesn't...

30 January 2021 5,482 3 View

STS Score - Excel sheet calculator?

I'm looking for an excel sheet to calculate the STS Score. From the website http://riskcalc.sts.org/stswebriskcalc/calculate. Does anyone have a worksheet to calculate the STS Score?

25 January 2021 958 4 View

Why would the TGA curve show weight gain?

Hello, I have several TGA curves of different compounds showing an odd weight gain/bump. The attached file is an example of some of these curves. I read oxidation can cause this if the purge gas...

21 January 2021 957 3 View