I will propagate pET-22b(+) from Novagen in TOP10(invitrogen) and then transform this plasmid to BL21(DE3) for protein expression
Top10 is delta recA1, delta endA1 E. coli and good strain for maintenance and propagation of plasmid vectors. Yes, you can use it in place of DH5 alpha.
Yes.
of course u can
My protein is expressed at 20C for 18h with 0.1mM IPTG Anyone can suggest optimal OD for induction?
10 November 2015 3,899 6 View
Which vectors are effective for secreted proteins from E. coli cell into medium?
10 November 2015 2,717 1 View
What is the optimal IPTG concentration and incubation time after induction? Normally, My protein were induced with 0.5mM at 25'C for O/N but now I have limitation about shaker, so i'll to try new...
08 September 2015 1,054 4 View
I wonder... What happen when express rare codon in E. coli? and Can be express full length protein even contain rare codon? ^-^
07 August 2015 3,910 0 View
06 July 2015 9,649 5 View
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25 February 2021 9,761 1 View
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24 February 2021 6,310 3 View
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24 February 2021 5,749 3 View
Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...
23 February 2021 7,429 1 View