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I will propagate pET-22b(+) from Novagen in TOP10(invitrogen) and then transform this plasmid to BL21(DE3) for protein expression
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Which vectors are effective for secreted proteins from E. coli cell into medium?
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My protein is expressed at 20C for 18h with 0.1mM IPTG Anyone can suggest optimal OD for induction?
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What is the optimal IPTG concentration and incubation time after induction? Normally, My protein were induced with 0.5mM at 25'C for O/N but now I have limitation about shaker, so i'll to try new...
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I wonder... What happen when express rare codon in E. coli? and Can be express full length protein even contain rare codon? ^-^
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My protein show correct size but very light on SDS-PAGE. In addition, Other size band is more bulky than my protein. I'm express in BL21(DE3) at 25'C for 18hr after added 0.5mM IPTG because...
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