Giggsy Matinny

6 Questions 2 Answers 0 Followers

Questions related from Giggsy Matinny

Could I use the TOP10 instead DH5alpha for pET-22b(+) plasmid transformation?

I will propagate pET-22b(+) from Novagen in TOP10(invitrogen) and then transform this plasmid to BL21(DE3) for protein expression

01 January 2016 2,954 3 View

Can anyone suggest commercial vectors for secreted recombinant proteins to medium?

Which vectors are effective for secreted proteins from E. coli cell into medium?

11 November 2015 2,764 1 View

Which is the optimal OD for protein induction?

My protein is expressed at 20C for 18h with 0.1mM IPTG Anyone can suggest optimal OD for induction?

11 November 2015 3,950 6 View

How do I express a secreted protein at 30-37’C?

What is the optimal IPTG concentration and incubation time after induction? Normally, My protein were induced with 0.5mM at 25'C for O/N but now I have limitation about shaker, so i'll to try new...

09 September 2015 1,108 4 View

Did anyone express rare codon in BL21DE3?

I wonder... What happen when express rare codon in E. coli? and Can be express full length protein even contain rare codon? ^-^

08 August 2015 3,961 0 View

Why my protein give a very light signal on SDS-PAGE?

My protein show correct size but very light on SDS-PAGE. In addition, Other size band is more bulky than my protein. I'm express in BL21(DE3) at 25'C for 18hr after added 0.5mM IPTG because...

07 July 2015 9,705 5 View