What are the parameters to assess the effectiveness of online teaching?

Assessment of online teaching

22 October 2020 2,897 23 View

How to design of PID controller using ANN?

27 March 2020 5,699 3 View

Hi, I would like to sense the Fe(III) in water, therefore how can I overcome the pH interference?

when adding the Fe compound in the water it gets deprotonated and from strong acid. 1.adding base leads to salt formation 2.adding buffer solution leads to Fe concentration change.

11 March 2020 1,785 3 View

What are the useful packages of i-vector/x-vector extraction in python?

Hi all, Recently, I have involved in the speaker language identification using i-vector /x-vector based approach. Kindly share any useful packages links in python to compile this research work....

04 February 2020 1,591 2 View

How to use neural network in MPC?

I implemented model predictive control for PMSM in Matlab. I want to incorporate neural network in my work. My system is MIMO system. Give me some suggestions.

14 January 2020 3,542 2 View

What are the essential areas in life long learning?

Areas in life long learning

31 December 2019 9,934 5 View

How to measure the cavity present in an organic molecule ?

Dear researcher, I wish to learn how to measure a cavity or cavity like arrangements present in an organic molecule. For proteins, many more online websites and softwares were available to...

24 August 2019 7,407 2 View

I am interested to work in Image processing with automation application, looking for tools and resources if any?

Image processing with automation application

11 August 2019 1,945 3 View

IGD indicator, GD indicator python or julia (MOEA)?

Hello all, Does anybody know any framework/code for IGD indicator, GD indicator in python or julia programming?.Also Is it ok to use a hypervolume indicator when it is biased with respect to...

30 July 2019 2,087 3 View

Which Services Are Provided By Window Azure Operating System?

operating system

08 March 2019 1,131 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

Best solvent for L-DOPA extraction and HPLC analysis?

I'm working on Mucuna pruriens and want to quantify L-DOPA. Could someone please suggest the cheap and best solvent for the extraction and the solvent system that has to be used for its...

20 February 2021 6,058 2 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

I have been reading a lot about PCR and still I am ending confused. I need to know the difference between RT-PCR, real-time PCR, qPCR, RT-qPCR ?

I Know that RT-PCR is Reverse Transcription PCR when the starting material is RNA. Going for reverse transcription first (RNA to cDNA) and then it could be conventional PCR with the need for gel...

11 February 2021 6,852 4 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Can I heat my ELISA at 37C?

Hello, I am conducting a sandwich-type ELISA. It is to see if small molecules can interfere with a protein-protein interaction. As this interaction would occur in the human body, may I heat...

31 January 2021 4,271 3 View