I am performing the synthesis of a protein-drug conjugation. After the conjugation, how do I calculate the concentrations of the protein and drug using UV? Kindly provide examples of calculations.
Hello, I am currently working on isolation of Clostridium perfringens type D from the feacal samples of sheep. I am getting pcr positive from clinical sample but I am facing problem in isolation...
09 March 2024 7,428 1 View
Is there any standards in American Petrochemical Institute available similar to pharmacopoeias? for the chemicals used in oilfield industry
15 September 2023 1,955 0 View
Hi all, I am trying to model a contact problem in DIANA FEA. I went through the DIANA manual and found that DIANA has contact elements which imposes the contact constraint. But there aren't any...
02 August 2023 7,405 3 View
I want to know whether any possible ways to charge the battery using supercapacitors with the help of converters. if yes what type of converters are prefered to charge the battery?
16 July 2023 154 1 View
I need to estimate catalase using hydrogen peroxide, sodium potassium phosphate buffer.
08 March 2022 7,118 1 View
single phase h bridge inverter
25 August 2021 6,751 1 View
Using pymol to visualize cyclic peptide. The cartoon representation of cyclic peptide is missing at the head to tail linkage which when represented with line and stick representation is...
04 August 2021 3,651 2 View
Good morning Sir/Madam, I want to use hardware of 1. PMSM with encoder. 2. PMSM controller 3. PMSM driver. all three must be compatible with each other. Please suggest any company product. I...
21 April 2020 6,719 0 View
In India we have tradition of keeping many groceries under the sun on terrace or exposed floor. Now due to covid situation, whatever delivered mainly and majorly being groceries,, we need to...
11 April 2020 6,619 3 View
Sir, How to design Neural Network based PID controller only with input and output data of conventional PID contorller in MATLAB simulink.
27 March 2020 6,106 3 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...
24 July 2024 8,696 0 View
For a partition of an integer \lambda, what does the difference \lambda_j - \lambda_{j+1} represent in relation to the conjugate partition, , with \lambda_j being the j-th element of \lambda?
23 July 2024 8,008 1 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View
How to calculate percentage cumulative drug release when, Total volume of dissolution media = 250ml Added formulation was equivalent to 10mg of drug Sample withdrawn = 1ml each time Sink condition...
15 July 2024 4,192 1 View