I have tried to isolate platelet total RNA from the human and sheep blood using TRIzol reagent. After isolation of platelets from the blood samples, total RNA extracted, and quantified using NanoDrop. The RNA concentration was around ~180-230 ng/ul ( 260/280 = ~1.87). The integrity of total RNA was assessed on an agarose gel, but I did not find any RNA bands over the gel except ladder. What could be the possible reason for not appearing RNA bands/smears on the gel? Suggestions and guidance will be highly appreciated.
I have attached a few papers which make use of TRIzol or acid-guanidium-phenol chloroform for RNA extraction from platelets which gave a yield of 20 ug of total RNA/ 30ml of blood. Hope this helps!
Article Quantitative isolation of RNA from human platelets
Article A Rapid and Efficient Platelet Purification Protocol for Pla...
Article Effects of pathogen reduction systems on platelet microRNAs,...
Thank you Joyleen Maryann Fernandes for the useful information and papers!
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