Could anyone tell me how to determine the level of reducing sugars?

I am working on parboiled rice, For checking the effect of parboiling on rice color I have to determine the level of Reducing sugars.

06 July 2019 3,768 3 View

Could anyone give me the TNBS method for measuring Free alpha Amino Nitrogen?

I am working on parboiled rice, after parboiling I have to check the level of free alpha-Amino nitrogen to determine the effect of steaming on rice color. Now I need the details about this method....

06 July 2019 3,546 1 View

Could anyone tell me how can I get the level of Free alpha-Amino Nitrogen (FAN) from Rice flour?

I am working on parboiled rice, after parboiling I have to check the level of free alpha-Amino nitrogen to determine the effect of steaming. Now which method should I follow, If possible kindly...

06 July 2019 2,113 4 View

How to maintain same moisture content???

For parboiling rice I have to follow three steps, soaking(A), steaming(B) and drying(C). In my experiment I have designed like this- 1.A,B,C 2.B,C 3.A,C to check the effect of these steps on rice...

04 May 2019 3,511 3 View

Can anyone tell me about the optimal temperature, time and a good method for rice parboiling(soaking,steaming,drying)??

For my experiment I have to parboil little amount of rice but I am confused about which method and optimization should I follow. It would be kind if you give me some suggestion or suggest me a...

04 May 2019 5,620 5 View

Could anyone suggest me for measuring and calculating rice color which method should I follow?

01 January 1970 6,312 7 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

Why does the sum of reaction forces not match the force component of a Free Body Cut in Abaqus?

I am using Abaqus to simulate a plate with in-plane loading. The boundary conditions allow the right edge to move up and down, the displacements on the left edge are fixed. There is no rotation...

23 February 2021 711 3 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View