Could anyone suggest me any good method for measuring the Furosine level of my rice sample?

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Could anyone tell me how to determine the level of reducing sugars?

I am working on parboiled rice, For checking the effect of parboiling on rice color I have to determine the level of Reducing sugars.

06 July 2019 3,873 3 View

Could anyone give me the TNBS method for measuring Free alpha Amino Nitrogen?

I am working on parboiled rice, after parboiling I have to check the level of free alpha-Amino nitrogen to determine the effect of steaming on rice color. Now I need the details about this method....

06 July 2019 3,654 1 View

Could anyone tell me how can I get the level of Free alpha-Amino Nitrogen (FAN) from Rice flour?

I am working on parboiled rice, after parboiling I have to check the level of free alpha-Amino nitrogen to determine the effect of steaming. Now which method should I follow, If possible kindly...

06 July 2019 2,209 4 View

How to maintain same moisture content???

For parboiling rice I have to follow three steps, soaking(A), steaming(B) and drying(C). In my experiment I have designed like this- 1.A,B,C 2.B,C 3.A,C to check the effect of these steps on rice...

04 May 2019 3,627 3 View

Can anyone tell me about the optimal temperature, time and a good method for rice parboiling(soaking,steaming,drying)??

For my experiment I have to parboil little amount of rice but I am confused about which method and optimization should I follow. It would be kind if you give me some suggestion or suggest me a...

04 May 2019 5,741 5 View

Could anyone suggest me for measuring and calculating rice color which method should I follow?

I am working on parboiled rice color. I am a new learner in this arena. Actually I have to calculate data based on three parameters, brightness(L), redness(a), yellowness(b) for measuring ΔE ....

01 January 1970 6,403 7 View

Is skin yellowness an numerical or ordinal variable?

I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...

11 August 2024 4,793 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Why applying a saved template of a bar chart in a different/new one doesn't take effect in SPSS?

Using SPSS, I made my edits in one bar chart — e.g., font type and size, hiding grid lines, and colours, namely: blue, orange, green, purple, and grey for 5 bars, respectively, etc. — and I saved...

29 July 2024 1,981 1 View

How to estimate sample size for GWAS of continuous and discrete traits? What are the pre-requisites?

Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color

28 July 2024 286 0 View