Is HBsAg negative result enough to recommend HBV vaccination? Is Anti-HBc assay important at all before taking vaccine?

This is because I am aware of a patient who was HBsAg negative and thereafter had his three doses of HBV vaccine only to test positive to both anti-HBs and anti-HBc four years later in a...

14 December 2019 3,438 17 View

How can Machine Learning count how many trees in an unlabeled land image?

I have multispectral images taken with the drone. I'm looking for an algorithm that can do tree counting and tagging through these images. I need to use python for this job and I need your...

05 December 2018 9,800 13 View

How can I programme a temperature chamber, water bath or heatmat to fluctuating temperature?

I am proposing an experiment to incubate soil under various temperature treatment to reflect Temperature fluctuation at various temperature steps without using environmental chambers or oven.

26 November 2018 7,549 3 View

What is the minimum length of unknown sequence that can be amplified using FirstChoice RLM Race Kit?

I am working on a non-model plant. The sequence of my organism does not exist. I have a RT PCR product that i amplified with primers of the plants within same family. The length of my amplicons...

24 May 2017 5,433 3 View

What are the most harmful chemicals in hydraulic fracturing flowback waters ?

There are hundreds of very harmful chemicals at this process i have to know which are most harmful.

24 May 2017 2,111 3 View

My cq values of target genes are lower than ones reference gene. What should i do?

13 February 2017 996 5 View

Dissolving of Vinclozolin for using for cell culture?

I tred to dissolve vinclozolin for cell culture use. I dissolved it in DMSO. There was no problem in this step. For further dilution, I was used full medium (DMEMF12+FBS), and water, seperatly....

24 October 2016 3,760 1 View

Is there any accurate equation which relates bicarbonate concentration to pH ?

I measured the pH of bicarbonate solution by a pH-meter, and I also calculated the pH of the same solution using the quantitative relationship between pH and pOH. However, they differed a lot. Can...

28 March 2016 8,868 2 View

How do I discover unknown SNPs with KASPar genotyping system?

We want to discover SNPs in our breeding materials. Cooperation of KASPar and Fluidigm is succesful to identify SNPs, but if we dont know SNPs how we can discover through Kaspar system?...

11 August 2015 7,918 4 View

Molecular weight or exact mass in LC-MS ?

I have found a molecular ion of m/z 140 in LC-ESI(-)-MS. Does it mean that the molecular weight of my target compound is 141 or the exact mass of my target compound is 141? I have asked this,...

01 October 2012 1,025 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View