Hello everyone!
I'm searching for a good chromatin immunoprecipitation kit.
I used Sigma Imprint Ultra ChIP kit, but had bad results (including no signals in positive controls).
It's desirable to include positive control Abs.
May be somebody use a good kit, and have good results with it?
Thanks in advance!
May be somebody checked "MAGnify™ Chromatin Immunoprecipitation System"?
And what about "17-10086 | EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit"?
Milipore ChIP kit is really working great and convenient.
http://www.emdmillipore.com/US/en/product/EZ-Magna-ChIP%E2%84%A2-A---Chromatin-Immunoprecipitation-Kit,MM_NF-17-408
http://www.protocol-online.org/forums/uploads/monthly_02_2009/msg-6761-1233506503.ipb
I'm successfully using the SimpleChIP Kit from Cell Signaling. It includes positive and negative control antibodies and primers and was always working finde for me :-)
Hi Evgeniy,
You can have a look at Diagendoe ChIp kits at https://www.diagenode.com/categories/chromatin-immunoprecipitation;
I can advise more precisly depending on what you are lloking for (histones, Tfs, number of cells per IP etc). All our kits has contorl Ab and primers for positive and negative loci. We have a very strong expertise in Chromatin IP and propose a whole solution for chIp, including instruments for chromatin prep, Abs , cHip kit and libraries prep kits.
Being from Saint-Petersburg, I will do my best to advise:)
Thanks a lot to everyone!
We bought a kit from Milipore.
Hello Irina! We needed a kit for TF. I checked for it at Diagenode web-site, and found iDeal ChIP-Seq kit, but it's rather expensive, and we don't need ChIP-seq yet, so we'll try Milipore first. Thanks!