14 June 2014 3 9K Report

We are analyzing the effects of Hedgehog signalling on B cell development.

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Experts on orchid viruses for an article for home gardeners?

I am writing an article for my blog regarding orchid viruses aimed at home gardeners. It will include details of home testing kits their use and accuracy. I also want to include details about...

26 June 2024 7,422 1 View

Job Evaluation - what is the key advantage / key disadvantage of point-factor rating?

I am look at Job evaluation and it feels that the advantages of point-factor rating seems to also be the disadvantages - for example it is objective, but also subjective

14 May 2024 4,747 0 View

Which questionnaire can be used to investigate which leadership style an employee prefers?

For my thesis, im doing research in finding the leadership style that is the best match with organization X to maintain work engagement. For this research I'm going to interview 10 employees and...

19 March 2024 539 0 View

Who is researching trauma right now, specifically childhood trauma?

Hi! I am graduating with my masters in Marriage & Family therapy the end of February & want to continue my education with a PhD. I applied to Florida State but that did not work out. What...

07 February 2024 3,341 2 View

Which LAMP kit works better: NEB or ThermoFisher?

Hello, I'm currently in need of performing Loop-Mediated Isothermal Amplification (LAMP). There are modifications in both the Bst DNA polymerases from NEB and ThermoFisher. Has anyone had...

31 January 2024 2,534 1 View

What is recent development/algorithms in elliptic curve cryptography for image encryption?

Image and text encryption

26 January 2024 1,223 4 View

Is anyone aware of a set of accessible anonymised free text clinical records related to primary care?

I am looking for access to some publicly (or cheaply) available free-text clinical records, to help with the development of an AI tool. I am aware of CPRD (too expensive, not free text) and...

15 January 2024 7,535 1 View

Prostate Cancer Cell Lines from Black / African origin patients?

Hello all. We are trying to get access to prostate cancer cell lines that were derived from patients who were Black or of African descent. There are numerous relevant cell lines reported in the...

07 December 2023 4,969 3 View

What type of sample prep is needed for SDS-PAGE electrophoresis of plasma?

I mixed EDTA mouse plasma with standard gel sample buffer and got very smeary image on Western with no distinct bands. Is there something in the plasma that needs to be removed before I run gels?

06 December 2023 8,966 1 View

What is this contamination?

I've never seen anything other than bacteria and this doesn't look like anything I can find on Google! Is it fungus?? This is a plate of neurospheres if that helps.

29 November 2023 7,553 2 View

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Can I use a HisTRAP column for affinity chromatography?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

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