Could Anyone please tell me the protocol of making 18% SDS-PAGE Gel?

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How can one know that BL21(DE3) is ready to express the protein of interest?

I had made BL21(DE3) cell competent.And for ensure that i had done transformation which gave many many colonies.So i start to do protein expression with this cell.But I can't understand it is not...

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Apart from using red light as a source to mimic DD conditions to Drosophila melanogaster to study the behaviour can any other light source be used ?

behavioural study of Drosophila melanogaster in DD conditions

08 February 2024 2,608 0 View

What are the suggestions on the esterification between dodecanol and poly(ethylene glycol)–poly(2-methyl-2-carboxyl-propylene carbonate?

In the last few months, trying to conjugate the dodecanol (DC) chain with the carboxylic units (30 units) of poly(ethylene glycol)–poly(2-methyl-2-carboxyl-propylene carbonate) via several...

29 December 2023 3,645 0 View

How could I Impart the tensile strength of thermoset (PF) polyurethane?

Here phenol formaldehyde-based resin is used to react with Polyisocyanate but the problem is a low tensile strength value. How could I get a Good Tensile strength? NOTE: 1: Phenol formaldehyde is...

06 July 2022 2,191 2 View

Here in this Tafel curve I have arranged the system rate of corrosion in the decreasing order (RED<GREEN<BLUE<BLACK) Please rectify me if I am wrong?

Corrosion rate (mpy) = 3.27x icorr x Ew /d --------------------------------------------(i) Where, Ew-Equivalent weight of mild steel, d is the density of mild steel. Is it the same formula for...

15 November 2021 9,868 6 View

How to compare performance of different optimization method without any benchmark?

Dear researchers, I've a selection problem, in which I need to select the top N items from a given list. The selection should be optimised based on some weighted selection criteria. I've used...

31 August 2021 2,424 11 View

In pbc calculations for impurity in a supercell, how to minimise the effect of impurities from surroundings supercell images?

How to minimize the effect of impurities from surroundings supercell images on our model supercell? My concern is about the surrounding periodic supercell images where also the modeled impurity...

06 August 2021 1,316 5 View

What besis set should I used to geometry optimization to see hydrogen bonding between water and one organic molecule?

I am using Orca 4. What besis set should I used? The molecule contain carbon, nitrogen and hydrogen atom.

30 June 2021 4,650 3 View

Which statistical test is appropriate to analyse Likert Scale data?

Recently, I conducted a research survey based on 5 points Liker scale data. All the options in every research-based questions are correct. The options do not depend on each other and are separate...

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Why Drug Addiction Is a Social Problem?

A common misconception surrounding drug addiction is that it solely affects the life of the user. However, the idea that they aren’t hurting anyone except for themselves is an excuse that many...

23 September 2020 3,059 12 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What is the cause of this aggregation just bellow 70 kDa mark?

Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...

21 July 2024 5,128 4 View