Nanotechnology has emerged as a new research field and has a broad view multidisciplinary approach. In this context, I started studying the occurrence of nanoparticles in plants and their effects on the ecosystem. In this regard I need some healthy tips.
UV-Visible spectrum analysis in samples..
XRD (X-ray Diffraction) analysis
FTIR (Fourier Transform Infrared Spectroscopy) analysis
Transmission Electron Microscopy (TEM) analysis
these are basic approaches for nanoparticles detection in samples..
DLS, TEM and FTIR are the characterisation methods presence of nanoparticles.to find the
I will be pleased if you people will suggest some chemical methods also for testing the presence of nano particles in plants
To the best of my knowledge there is no chemical method available for quantifying nanoparticles in plants. However, one can acid digest plant material to find the total content of metal (if you are referring to metal NPs) of your choice using AAS/FAAS analysis. Many a times when we digest plant material with Au-nanoparticles, we see the formation of glittering gold metal. In other words, we cannot detect total quantity of gold in plants containing gold NPs using AAS.
It is difficult to extract nanoparticles from plant tissues as they may be bound with cell wall material and several other complexes. They may be present in chloroplasts/plastids, vacuoles, mitochondria etc. There are several types reducing agents (which include sugars, organic acids, phenolics, aminoacids, glutathione, starch, proteins etc.) in living cells that can promote generation of nanoparticles from metal ions. Therefore, we have been recoding their presence in several parts of the cell. We have also been making efforts in extracting out the nanoparticles from plants. You may look at some of our publications, where we showed potential of live plants and isolated chloroplast to generate metal nanoparticles.
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