I am working on the cell-tracking imaging with macrophage Raw 264.7 now, we need to know the migration ability before labeling them. From the literatures, some people used 8um pore size, and some used 5um, we do not know what to choose... And, what chemotaxin are you using? Can we used CSF-1?

And, is the migration ability different between the cells at different passage number?

Thank you for the help in advance!

Yanrong

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