Cell Culture Protocol for 67NR and 4T07 cells?

Hello there! My lab has recently been (very graciously) gifted an array of cell lines, of which include 67NR and 4T07 cell lines. While I've found a decent amount of background and culturing...

02 February 2021 2,590 3 View

How can I calculate Standard Deviation using DataWarrior?

I am analyzing about 500 results in DataWarrior and I need to calculate the mean value and the SD. I managed to calculate the mean value using excel, but it I couln't find a way to simply...

25 October 2020 9,318 3 View

Computer vision / machine learning techniques for "blob" tracking and prediction?

My research involves tracking a dye plume in the coastal zone using computer vision techniques, and potentially making predictions using machine learning. If anyone knows any resources that come...

15 April 2020 6,507 2 View

Escalating dose regimen with osmotic mini pump?

Hi everyone, I wonder if someone has ever used osmotic mini-pump for an escalating dose regimen. I read that some iPRECIO osmotic mini-pump are programmable and therefore can be used for infusion...

03 April 2020 9,009 3 View

Is there anyone that have a trasfection protocol optimized for suspension cell culture?

We work with WIL2 cell line and want to trasfect mirna mimic and inhibitor. We tried first with lipofectamin and than electroporation (using amaxa nucleofector, U-001 program), none of that works....

27 March 2017 4,176 4 View

IWP-2 inhibitor

16 February 2016 1,730 4 View

Is there any solvent to solve SEBS blend?

I need to know the amount of oil present as plasticizer, so I would like to know il i can dissolve SEBS in one solvent and then recrystallyze it in another solvent.

30 September 2015 9,013 2 View

Could you help me with a Capitellidae identification?

Hi, I'm working with samples of the Portuguese Continental Shelf; I found few specimens of Capitellidae with 11 thoracic segments, in which the first is achaetigerous and the other 10 with only...

19 July 2015 1,087 4 View

Is anyone working with PK15 cell line free form circovirus?

I would like to find PK15 cell line free of Circovirus to attempt PCV2 isolation.

06 May 2015 6,617 2 View

Does any body explain why there is a superheater stage in the organic rankine cycles (ORC)?

I am interested in ORC-system. If I look to the hp-diagrams of the main working fluid they do not need a superheating stage, but in reality I saw that in many ORC the fluid is also superheated....

18 February 2015 10,018 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View