Has anyone had experience setting up a Getinge 500 mL mini-bioreactor?

03 May 2024 1 8K Report

Just wondering if anyone has any experience in setting up a Getinge mini bioreactor? More specifically, autoclaving the reactor, the gel pH sensor, and the dO2 sensor.

I am having trouble understanding how to use the pH probe and "pressurize" in the autoclave before first use. The pH probe has a lifecycle of 10-15 autoclaves. Would I have to bathe the probe in ethanol as a method of sterilizing the probe between cell cultures?

Any advice is welcomed! Thanks so much in advance!

Prabakaran Krishnamurthi

The probes will have to be sterilized along with the medium in the reactor after the sterilization cool the reactor to the required temperature and calibrate both pH and DO probes.

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