Cell Culture Protocol for 67NR and 4T07 cells?

Hello there! My lab has recently been (very graciously) gifted an array of cell lines, of which include 67NR and 4T07 cell lines. While I've found a decent amount of background and culturing...

02 February 2021 2,590 3 View

How can I calculate Standard Deviation using DataWarrior?

I am analyzing about 500 results in DataWarrior and I need to calculate the mean value and the SD. I managed to calculate the mean value using excel, but it I couln't find a way to simply...

25 October 2020 9,318 3 View

Computer vision / machine learning techniques for "blob" tracking and prediction?

My research involves tracking a dye plume in the coastal zone using computer vision techniques, and potentially making predictions using machine learning. If anyone knows any resources that come...

15 April 2020 6,507 2 View

Escalating dose regimen with osmotic mini pump?

Hi everyone, I wonder if someone has ever used osmotic mini-pump for an escalating dose regimen. I read that some iPRECIO osmotic mini-pump are programmable and therefore can be used for infusion...

03 April 2020 9,009 3 View

Is there anyone that have a trasfection protocol optimized for suspension cell culture?

We work with WIL2 cell line and want to trasfect mirna mimic and inhibitor. We tried first with lipofectamin and than electroporation (using amaxa nucleofector, U-001 program), none of that works....

27 March 2017 4,176 4 View

IWP-2 inhibitor

16 February 2016 1,730 4 View

Is there any solvent to solve SEBS blend?

I need to know the amount of oil present as plasticizer, so I would like to know il i can dissolve SEBS in one solvent and then recrystallyze it in another solvent.

30 September 2015 9,013 2 View

Could you help me with a Capitellidae identification?

Hi, I'm working with samples of the Portuguese Continental Shelf; I found few specimens of Capitellidae with 11 thoracic segments, in which the first is achaetigerous and the other 10 with only...

19 July 2015 1,087 4 View

Is anyone working with PK15 cell line free form circovirus?

I would like to find PK15 cell line free of Circovirus to attempt PCV2 isolation.

06 May 2015 6,617 2 View

Does any body explain why there is a superheater stage in the organic rankine cycles (ORC)?

I am interested in ORC-system. If I look to the hp-diagrams of the main working fluid they do not need a superheating stage, but in reality I saw that in many ORC the fluid is also superheated....

18 February 2015 10,018 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

How to obtain regions around the person bounding boxes(excluded the detected people in scene)?

Hi all. I have a set of coordinates of bounding boxes for AVA Dataset. This dataset is used in action recognition. My goal is to get the areas around the people (people are inside boxes) and do...

13 February 2021 2,538 2 View

How to deal with multidimensional predictor in multiple regression?

I am investigating the influence of Trait Emotional Intelligence(TEI) and Communication styles (specifically Precision and Expressiveness styles) on Academic performance. I am now in a fix as to...

10 February 2021 7,630 3 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View