What are the basic differences in biochemical properties of Bacillus subtilis and Bacillus pumilus?

I have sequenced a bacterial strain which showed 100% similarity with both B. subtilis and B. pumilus with same hits. However, both of them are gram positive and hence I need to know the...

09 October 2016 193 4 View

Can anybody help me in getting some recent literature's on the use of PGP microbes along with biochar for enhancing crop productivity?

I am in search of some recent literature's on the use of PGP microbes with biochar for increased crop productivity and their future prospects. Thanks in advance.

08 September 2016 1,643 7 View

Bacillus strains could produce 2-ketogluconic acids or not?

In a recent literature survey I could find a reference stating that they could produce this organic acid but I am not sure in this regard. I will be very much grateful if somebody having expertise...

07 August 2016 5,694 3 View

Is it possible to change or adjust pH of an entire plot of land? If so how?

07 August 2016 5,866 22 View

Can anyone help me getting some literature's on qualitative screening of Gibberellin production by bacteria?

I need to screen out some PGPR isolates based on qualitative screening before moving towards quantitative assay. But during literature survey I am not able to get literature's on qualitative...

01 January 1970 8,197 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View