HPLC with gradient elution method is used for the determination of the synthetic phenolic antioxidants (SPAs) propyl gallate (PG), tertiary butyl hydroquinone (TBHQ), butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT) in food items. It needs C18 column served as the stationary phase; the gradient elution can be formed by acetonitrile and water:acetic acid (1%). The UV detector needs to set at 280 nm. Under the recommended conditions, separation of the four SPAs can be  achieved in less than 8 min.Recoveries of the SPAs in different types of food items (cooking oil, margarine and butter, and cheese) can be made. For this purpose  SPAs are extracted with methanol/acetonitrile (1:1, v/v) and are  subjected to vortex/ultrasonic treatment. The extracts is kept in a freezer (∼2 h) to precipitate co-extracted components.

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http://www.sciencedirect.com/science/article/pii/S0308814610011040