I am currently performing a mouse model of wound healing. The process is to make the wound, apply and suture the silicone splint, put the tegaderm and apply topically the treatment every three days. My problem is that on the 7th day wounds have purulent exudate. All the material is autoclaved and aseptic conditions are maintained. Has anyone had the same problem? Could an antibiotic be applied?
On the other hand, I want to test an extract using Labrasol as vehicle, Does someone use it or know about what I can use?
In my opinion, if all infections in the experiment, I think you need to control the cleanliness although the tools are sterilized. However, it may still not clean enough, but the mice are infected 1-2 in the groups, you should be separated from the room, and treat with topical antiseptic. Next, you should find how infection. The purulent exudates wound is bad wound healing, you can apply antibiotic if it yours design model.
What is organism cultured? I suspect the silicone splints are contaminated. Try doing a sham operation leaving out the silicone. If infection is still a problem, then try culturing your suture material. I agree with Dr. Kwansang, if you are getting 100% contamination, then your equipment may be contaminated. Try also checking your autoclave.
Sincerely,
Alan Coulson
Thank you very much for your help. Finally it has already solved with better aseptic conditions.
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