How can I get the copy number of DNA in the sample by using qPCR, I tried relative standard curve.
Hi Dr. Khwam
To get the copy number of a DNA fragment..you'll have to use 'absolute quantification method' ...follow the guidelines of machine you are using for qPCR analysis
bye
Hi Dr Ajay,
Thank you so much, yes I did recently absolute Standard Curve after cloning my target primers in plasmid and transformed it on E. coli.
Kind regards
Nice to hear you have already worked it out..
Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.
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