Generating an efficient lentivirus/AAV-producing HEK293/FT or lentiviral particle?

Hi experts, I am trying to produce lentivirus/AAV-producing HEK293/FT? I have a few questions regarding the protocol and results. I followed multiple sources, but the one I really followed is...

06 July 2018 4,080 4 View

What is the best age of mice to start stereotaxic injection and the best time to see highest expression?

Hi Experts, Can you give me an advice on these critical conditions for stereotaxic injection in mice? 1. What is the best age to inject brain regions - striatum and substantia nigra? Based on my...

04 May 2018 2,486 3 View

Can I measure the DNA concentration with nanodrop after Chromatin Immunoprecipitation (ChIP)?

Hi Experts, I am trying to do ChIP qPCR assay. So I followed the protocol from EMD Millipore/Sigma Aldrich kit for ChIP assay. According to the protocol, at the end of ChIP, just use 2 uL of...

03 April 2018 7,732 6 View

Why do we transfect cell during proliferation and not during differentiation?

Hi Experts, I am trying to transfect LUHMES cells and I've seen different protocols for transfection. Before or after differentiation, which one is better? And why? I've tried transfecting the...

03 April 2018 5,921 4 View

How can I identify and eliminate my cell culture contamination?

Hi experts, I need your help in identifying this kind of contamination. It has been a pain in my experiment to see this unforeseen event. This appeared first during my transfection experiments....

08 September 2017 4,021 8 View

In Western blotting, what is your experience with Wet vs Dry Transfer Systems?

Hi experts, I need your advice when using Dry Transfer System for transferring proteins from gel to nitrocellulose membrane. Have you ever used home-made gels, nitrocellulose membrane, and filter...

06 July 2017 8,936 15 View

What is the problem in my submission of metagenomic sequences to NCBI SRA?

Hi, I recently upload raw sequence reads to NCBI Sequence Read Archive (SRA). I followed the steps: (1) Make BioProject, (2) Make BioSamples (3) Submit to SRA (Beta) using Aspera Connect. However,...

06 July 2016 2,616 3 View

What are the practical advantages and disadvantages of different protein purification methods?

10 November 2015 9,182 3 View

How do I determine if a peptide is cationic or anionic?

Hello Experts, I am still a beginner in this field, and I want to understand if a certain DNA or amino acid sequence is cationic or anionic? Is there a way (not experimentally), using...

10 November 2015 8,224 10 View

Can I use a microplate reader to detect fluorescence?

This is my first time to do experiment using fluorescent proteins. And I am wondering a cheaper way to detect and directly measure relative fluorescence units as a way of determining...

07 August 2015 5,129 7 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View