This is my first time to do experiment using fluorescent proteins. And I am wondering a cheaper way to detect and directly measure relative fluorescence units as a way of determining expression.
Of course, I can use Western blot and ELISA methods for the assay of my expression system, however, I am looking for cheaper ways to do this and I found that fluorescent microplate reading technique is a good and efficient way to do this.
However, in our lab, we have an old model of Bio-rad Model 680 micro plate reader for ELISA experiments. But I am not sure if it can measure fluorescence. The manufacturer discontinued the selling of this instrument but continues to support accessories and services. In their website, they also sell Interference filters, and I am wondering if it is possible to use this device? I am not sure if this device has both excitation and emission channels.
Here's a link to the device: http://www.bio-rad.com/en-kr/product/model-680-microplate-reader
The reader you mentioned is a "simple" ELISA reader. As ELISA is measured via absorbance detection, this reader cannot be used for fluorescence intensity measurements as it is able to only detect absorbance.
Thank you for that swift response! What specific kind of micro plate reader should I look for when I am browsing the description of different micro plate readers?
Nowadays there are very few fluorescence-only readers. The major part are multi-mode plate readers. However, these readers are modular so that you can have it only with fluorescence intensity and in case upgrade it with other detection modes in the future. So you should Google for “fluorescence measurement microplate reader” for instance. "fluorescence intensity" is the correct definition of the detection mode you need, abbreviated with FI. The easiest way to understand what a reader can measure is to check the reader specifications. You can find it on every manufacturer´s product page on the internet.
If you go for instance to the link I attached (the company I am working for), you can clearly see the capability (detection modes) of the reader. You should check if “fluorescence intensity” is mentioned under the detection modes and if a sensitivity is stated for “fluorescence intensity”. By this you are sure that you are looking for the correct reader. Fluorescence intensity is one of the basic detection modes so you have plenty of readers that offer it. I hope this helps you!
Thanks Tobias and Edward, this isn't an answer but an additional question for Tobias since you work for BMG LabTech GmBH. I need to know how much it would cost to purchase a simple to moderate "fluorescence measurement microplate reader" from your company. I need one in my small lab and I am desperately in need of a quote.
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