20 Questions 69 Answers 0 Followers
Questions related from Edward Alain Pajarillo
Hi experts, I am trying to produce lentivirus/AAV-producing HEK293/FT? I have a few questions regarding the protocol and results. I followed multiple sources, but the one I really followed is...
07 July 2018 4,207 4 View
Hi Experts, Can you give me an advice on these critical conditions for stereotaxic injection in mice? 1. What is the best age to inject brain regions - striatum and substantia nigra? Based on my...
05 May 2018 2,590 3 View
Hi Experts, I am trying to transfect LUHMES cells and I've seen different protocols for transfection. Before or after differentiation, which one is better? And why? I've tried transfecting the...
04 April 2018 6,021 4 View
Hi Experts, I am trying to do ChIP qPCR assay. So I followed the protocol from EMD Millipore/Sigma Aldrich kit for ChIP assay. According to the protocol, at the end of ChIP, just use 2 uL of...
04 April 2018 7,829 6 View
Hi experts, I need your help in identifying this kind of contamination. It has been a pain in my experiment to see this unforeseen event. This appeared first during my transfection experiments....
09 September 2017 4,106 8 View
Hi experts, I need your advice when using Dry Transfer System for transferring proteins from gel to nitrocellulose membrane. Have you ever used home-made gels, nitrocellulose membrane, and filter...
07 July 2017 9,004 15 View
Hi, I recently upload raw sequence reads to NCBI Sequence Read Archive (SRA). I followed the steps: (1) Make BioProject, (2) Make BioSamples (3) Submit to SRA (Beta) using Aspera Connect. However,...
07 July 2016 2,712 3 View
Hi Experts, I need your help. I need an updated online tool that can convert IDs from 1 database ID to another. I've been using bioDBnet website. However, I'm not sure if this is well maintained...
02 February 2016 7,814 2 View
Hello everyone,I just want to have a quick question (and hopefully a quick and brief response), what are the advantages and disadvantages of different protein purification methods, say ionic...
11 November 2015 9,283 3 View
Hello Experts, I am still a beginner in this field, and I want to understand if a certain DNA or amino acid sequence is cationic or anionic? Is there a way (not experimentally), using...
11 November 2015 8,306 10 View
This is my first time to do experiment using fluorescent proteins. And I am wondering a cheaper way to detect and directly measure relative fluorescence units as a way of determining...
08 August 2015 5,232 7 View
I've been designing primers for capturing promoter regions but I wonder this (it's not in the books). Concept 1: Promoters are -35 and -10 upstream their gene in the genome. Concept 2: Predicted...
06 June 2015 8,991 9 View
We have recently submitted a complete genome of an organism. I wonder if we can access and analyze pathways for the organism. I tried searching several GI and Genbank Accession Numbers into the...
03 March 2015 5,160 13 View
I am looking for a very detailed step-by-step protocol for performing stability check of recombinant plasmid DNA in Lactobacillus? Thanks in advance!
02 February 2015 4,090 4 View
I am looking for a method for rapid purification of heterologous proteins in Lactobacillus species. Are there any papers or book that you can recommend? I am currently constructing expression...
02 February 2015 10,064 2 View
I am very interested in studying programming on my own, but I don't know what should I focus on is it C++, Javascript, R, Python, Perl, Ruby? And can you recommend specific steps on how to study...
01 January 2015 2,019 5 View
Can you help me find a detailed method/protocol/paper that shows step-by-step walkthrough in checking the (1) plasmid copy number and (2) transformation efficiency in lactobacilli or at least...
01 January 2015 9,132 3 View
Okay, I have been constructing a plasmid shuttle cloning vector for a certain bacterium. First, I inserted the plasmid DNA from my original source and pUC19, then I put erythromycin gene. Now, I...
01 January 2015 846 10 View
Hi experts, Since RNA-seq with NGS technology is changing gene expression studies with great advantages. We still observe a lot of studies using microarray (i.e. Affymetrix Gene Atlas, etc.)...
01 January 1970 4,272 10 View
Hi Experts, I know that there is no perfect methodology for any experiment. But I am just wondering since I have been doing Flow Cytometry experiments and just curious for the best isolation...
01 January 1970 709 1 View