Edward Alain Pajarillo

20 Questions 69 Answers 0 Followers

Questions related from Edward Alain Pajarillo

Generating an efficient lentivirus/AAV-producing HEK293/FT or lentiviral particle?

Hi experts, I am trying to produce lentivirus/AAV-producing HEK293/FT? I have a few questions regarding the protocol and results. I followed multiple sources, but the one I really followed is...

07 July 2018 4,189 4 View

What is the best age of mice to start stereotaxic injection and the best time to see highest expression?

Hi Experts, Can you give me an advice on these critical conditions for stereotaxic injection in mice? 1. What is the best age to inject brain regions - striatum and substantia nigra? Based on my...

05 May 2018 2,570 3 View

Why do we transfect cell during proliferation and not during differentiation?

Hi Experts, I am trying to transfect LUHMES cells and I've seen different protocols for transfection. Before or after differentiation, which one is better? And why? I've tried transfecting the...

04 April 2018 6,004 4 View

Can I measure the DNA concentration with nanodrop after Chromatin Immunoprecipitation (ChIP)?

Hi Experts, I am trying to do ChIP qPCR assay. So I followed the protocol from EMD Millipore/Sigma Aldrich kit for ChIP assay. According to the protocol, at the end of ChIP, just use 2 uL of...

04 April 2018 7,813 6 View

How can I identify and eliminate my cell culture contamination?

Hi experts, I need your help in identifying this kind of contamination. It has been a pain in my experiment to see this unforeseen event. This appeared first during my transfection experiments....

09 September 2017 4,090 8 View

In Western blotting, what is your experience with Wet vs Dry Transfer Systems?

Hi experts, I need your advice when using Dry Transfer System for transferring proteins from gel to nitrocellulose membrane. Have you ever used home-made gels, nitrocellulose membrane, and filter...

07 July 2017 8,990 15 View

What is the problem in my submission of metagenomic sequences to NCBI SRA?

Hi, I recently upload raw sequence reads to NCBI Sequence Read Archive (SRA). I followed the steps: (1) Make BioProject, (2) Make BioSamples (3) Submit to SRA (Beta) using Aspera Connect. However,...

07 July 2016 2,695 3 View

Converting GI number to other Database ID, and extracting batch protein sequences?

Hi Experts, I need your help. I need an updated online tool that can convert IDs from 1 database ID to another. I've been using bioDBnet website. However, I'm not sure if this is well maintained...

02 February 2016 7,798 2 View

What are the practical advantages and disadvantages of different protein purification methods?

Hello everyone,I just want to have a quick question (and hopefully a quick and brief response), what are the advantages and disadvantages of different protein purification methods, say ionic...

11 November 2015 9,261 3 View

How do I determine if a peptide is cationic or anionic?

Hello Experts, I am still a beginner in this field, and I want to understand if a certain DNA or amino acid sequence is cationic or anionic? Is there a way (not experimentally), using...

11 November 2015 8,289 10 View

Can I use a microplate reader to detect fluorescence?

This is my first time to do experiment using fluorescent proteins. And I am wondering a cheaper way to detect and directly measure relative fluorescence units as a way of determining...

08 August 2015 5,214 7 View

Am I understanding genes and genomes correctly when it comes to promoters and terminators?

I've been designing primers for capturing promoter regions but I wonder this (it's not in the books). Concept 1: Promoters are -35 and -10 upstream their gene in the genome. Concept 2: Predicted...

06 June 2015 8,978 9 View

How can I do pathway analysis from a recently uploaded (complete) genome?

We have recently submitted a complete genome of an organism. I wonder if we can access and analyze pathways for the organism. I tried searching several GI and Genbank Accession Numbers into the...

03 March 2015 5,150 13 View

What is the most detailed protocol for plasmid stability confirmation?

I am looking for a very detailed step-by-step protocol for performing stability check of recombinant plasmid DNA in Lactobacillus? Thanks in advance!

02 February 2015 4,078 4 View

Are there any rapid protein purification techniques and identification methods for Gram-positive bacteria, particularly Lactobacillus sp.?

I am looking for a method for rapid purification of heterologous proteins in Lactobacillus species. Are there any papers or book that you can recommend? I am currently constructing expression...

02 February 2015 10,054 2 View

What is the best programming language to focus and study for Bioinformatics?

I am very interested in studying programming on my own, but I don't know what should I focus on is it C++, Javascript, R, Python, Perl, Ruby? And can you recommend specific steps on how to study...

01 January 2015 2,009 5 View

Is there a detailed protocol for checking plasmid copy number and transformation efficiency?

Can you help me find a detailed method/protocol/paper that shows step-by-step walkthrough in checking the (1) plasmid copy number and (2) transformation efficiency in lactobacilli or at least...

01 January 2015 9,120 3 View

Is there a problem with my plasmid construction protocol?

Okay, I have been constructing a plasmid shuttle cloning vector for a certain bacterium. First, I inserted the plasmid DNA from my original source and pUC19, then I put erythromycin gene. Now, I...

01 January 2015 832 10 View

Choosing between microarray and RNA-seq for gene expression studies and more?

Hi experts, Since RNA-seq with NGS technology is changing gene expression studies with great advantages. We still observe a lot of studies using microarray (i.e. Affymetrix Gene Atlas, etc.)...

01 January 1970 4,261 10 View

What is the best protocol for cell sorting from frozen brain tissues?

Hi Experts, I know that there is no perfect methodology for any experiment. But I am just wondering since I have been doing Flow Cytometry experiments and just curious for the best isolation...

01 January 1970 698 1 View