Continuous absorbency assays involving Beta-NADH - can anyone help?

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Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

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How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

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Christopher Barbieri

Are you monitoring the change in absorbance at 340 nm unpon oxidation/reduction of NADH/NAD? It's possible that at high imidizole concentrations you are seeing absorbance in this wavelength region. It's best to use freshly prepared imidizole since imidizole can be oxidized and show more UV wavelength absorbance. (That's why it turns brown). If you are not saturating your dectector (i.e. your OD

Gabriela Arias De la Rosa

Yes, I am monitoring the decrease of absorbance at 340 nm due to oxidation of beta-NADH. Thanks for your help.