Gabriela Arias De la Rosa

13 Questions 11 Answers 0 Followers

Questions related from Gabriela Arias De la Rosa

Will making a hydride acceptor more electrophilic speed up hydride transfer?

I have been searching the literature about this and I have not found an answer. If the atom that accepts a hydride is made more electrophilic, can this speed up the rate of hydride transfer?

30 July 2020 10,100 1 View

Can the energy displayed in Gaussian Calculation Summary be used to compare the stability between molecules?

I want to compare the stability of molecules that have the same number of atoms. I optimized these structures and calculated their vibrational frequencies. Gaussian09 gives a Gaussian Calculation...

19 July 2020 5,402 5 View

Virtual labs for weighing using an analytical balance and/or a quadruple beam balance?

I have found some virtual labs on how to do weighings using a triple beam balance but not on how to use an analytical balance or quadruple beam balance. If anyone knows of a website where I can...

27 May 2020 9,193 0 View

Electrostatic potentials from gaussian are always accurate when predicting protonation sites?

I'm using electrostatic potentials to analyze which sites are the most likely to be proton acceptors in a substrate. Based on these calculations, the site which I believe is the proton acceptor is...

09 December 2019 6,146 1 View

What is the best book for finding characteristics group frequencies in Raman?

I need to make some assignments in my Raman spectra and want to use a book to guide them.

22 November 2019 1,515 2 View

Is it possible for a protein to bind to a weak cation exchanger at a pH above its pI?

I'm reading about the schemes used to purify a protein from different sources. In one of these schemes, they used buffers with a pH (6.5) above the pI (5.7) of the protein to equilibrate a weak...

05 April 2019 6,036 3 View

Calculating buried surface of a ligand bound to a protein?

Is there a free tool to calculate the percentage of a ligand that is inside a protein? Thanks.

20 January 2017 9,050 5 View

What techniques can be used to determine whether a protein sample is properly folded?

I know NMR and X-ray crystallography can be used to determine the structure of a protein. Are there any other techniques that can be used to check whether a protein has native structure?

16 December 2015 7,530 5 View

Has anyone used a Cold-Shock Expression System employing the pCold vector in E. coli?

I am thinking in using this expression system to express dihydropteridine reductase. I want to know if anyone has used this system before and what advantages and disadvantages they have over other...

04 February 2014 8,469 4 View

Continuous absorbency assays involving Beta-NADH - can anyone help?

I want to know if imidazole concentrations of up to 250 mM or 1% triton x-100 can interfere with this assay.

29 October 2013 7,734 2 View

What is the best way to purify a N-terminal His-GST tagged protein?

I have purified His tagged proteins in the past, but I am a bit confused on how to purify doubly tagged proteins. I tried looking in the literature on how to do this, and I have not found anything...

13 June 2013 5,319 4 View

Which are the best books for learning theoretical chemistry?

a specialization in theoretical chemistry. Thus, I would like to know which books would help me acquire a solid

07 February 2012 5,875 12 View

What is the best chemical speciation software to determine the speciation of Citrate and Zinc (II) at different pHs?

I need to determine the protonation states the complexes form by citrate and Zn(II) have at different pHs.

31 January 2012 2,224 9 View