Does anyone know a protocol to induce inflammation in transwell Caco-2 cells? I'm having difficulty finding a strong and consistent model for that.
After 21 days of Caco-2 monolayer differentiation in transwell plates, my idea is to induce inflammation using LPS (1 microgram/mL) on the basolateral side. Studies however, present different ways of doing this: (a) by applying LPS to both sides (apical, basolateral) or (b) by applying LPS to only one of the sides (apical or basolateral). Also, many dosages are used by studies, varying from ng to micrograms.
Therefore, I'm very confused regarding which dosage of LPS to use and the compartment (apical, basolateral) of choice. If anyone has done this successfully, please let me know.
Thank you so much,
Hello
LPS is not a good way to induce inflammation in Caco-2 cell line. TNFa + IFN 50ng/ml each at basal side is a better way