Defining mixed model with repeated measures data?

HI all, I have been working with daily diaries data ( 6 emotions of which 3 positive and 3 negative) were assessed for 30 days. These observations (1level data) are then nested within subjects...

13 February 2021 1,810 3 View

High percentage agarose gel for better resolution?

Hi all, it might be a very basic question, but I need some opinions: I need to separate a 294 bp wild type PCR product from a 232 bp knock-out PCR product. I want to do DNA extraction out of the...

08 June 2020 9,557 9 View

What do you think of the impact of Covid-19 on faster acceptance of innovative healthcare technologies?

After this pandemic outbreak, nothing will be the same. Previously suspicious attitude toward accepting innovative solutions in clinical practice and long times for regulatory procedures and...

09 April 2020 7,691 10 View

Any experience with Health Informatics Journal?

We would like to publish something which is in the domain of Health Informatics, but several Journals have almost identical names. any suggestions, experience with publishing with those Journals...

22 February 2020 7,635 9 View

Do we need a standardization prior to publication of any machine learning application?

What do you think about so high accuracies reported in so many publications especially in medicine and psychiatry? Talking about overfitting and so frequent lack of internal and external...

07 October 2019 2,558 4 View

Is it possible to stain cells with Acridine orange/ethidium bromide in hydrogel 3D invasion assay?

I am planning to perform 3D invasion assay with spheroids in hydrogel and to visualise live/dead cells. Is it possible to visualise cells with AO/EB (besides CAM/EtHD) dyes? Thanks

18 October 2018 5,722 3 View

Can LPS be captivated by the tube if you freeze and subsequently thaw it?

15 January 2017 7,051 3 View

Does someone can recommend a good Elisa kit for mouse NTx (N-terminal telopeptide ) ?

Hello everyone, I need to measure NTx (N-terminal telopeptide) marker in a mouse serum. I would really appreciate if someone could share experience with this kind of ELISA kit, most importantly...

10 April 2016 5,458 1 View

What is wrong with my intestinal organoids?

I am trying to grow swine intestinal organoids from crypt preps. I have been doing it with mouse tissue for at least a year now. For the pigs, a few days after getting the crypts, there are...

05 April 2016 3,294 7 View

Does anyone know how to create nucleotide and amino acid deduced sequence visualization in an automated way?

I would like to generate a visualization similar to that you can see in the attached image (Mu et al., 2012, Fish and Shellfish Immunology Journal).

15 September 2012 7,903 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View