Why is my GOI not expressing after lentiviral transduction despite RNA increase and protein is detected in transfection?

Hi, I'm trying to create a stable overexpression line with EF1a-FlagGOI-IRES-GFP using lentivirus packaged with pxPAX2 and pM2G. After transduction of 293T with 8ug/ml polybrene, I was able to...

29 January 2021 1,878 4 View

How to correct a deviation in the molar ratio in an Isothermal Titration Calorimetry equipment?

Our equipment of ITC does not reach the molar ratio (N=1) of a titration of EDTA with Ca2+ in MES buffer pH 6. We have cleaned with DCN 90 and HCl pH 2, but the N value is very low, around...

10 November 2020 3,137 3 View

How can I obtain the cohesive energy density and solubility parameter for a crystal using the Materials Studio software?

Hello, I am currently analyzing properties of some minerals such as Bi2O3 by MD using Materials Studio, I need to obtain the cohesive energy density (CED) to obtain the solubility parameter,...

02 November 2020 4,356 1 View

In which solvent should I reconstitute mitoTEMPO?

Dear colleagues, I was wondering which solvent should I use to reconstitute mitoTEMPO, I've seen I could use water or DMSO but I'm not sure about the stability of the compounds in the respective...

18 May 2020 2,882 3 View

Does anyone knows if is there any open source repository of historical aerial photography that includes the north of Chile?

My PhD includes a remote sensing survey of the Atacama Desert using freely available satellite imagery to detect archaeological sites. So far, I have not found any source of historical aerial...

12 May 2020 1,383 8 View

Converting Large Chemical Formats to .pdbqt for AutoDock Vina?

I am trying to convert a large single file library (.sdf, .pdb) containing 50k+ molecules into a .pdbqt file for screening. I have tried using AutoDock Tools but it crashes upon trying to load the...

26 April 2020 5,020 6 View

Do you think the approach to sustainability and environmental issues will change for human society after this pandemic?

We need to be closer to the nature and don't look for money and other values.

29 March 2020 1,238 4 View

Which statistical analysis should I follow for analyzing the effects of two factors in two different cell types cultured in vitro?

Hello all! I have performed experiments involving two different cell types. These two cell types have been cultured in vitro and measurements have been performed after 4, 7 and 10 days of culture....

18 March 2020 6,337 10 View

Can Bacteria grow in DMSO 99%?

I used to test antiproliferative activities of chemical compounds dissolved in DMSO. I have been surprised when testing one of this compounds in HBL-100 cell line bacteria contamination appears. I...

13 February 2020 4,542 2 View

Does anyone know how many samples can I sequence with the minION basic starter pack ?

02 December 2019 3,228 1 View

According to a new research?

17 February 2021 2,372 3 View

Can any one provide information about medicine, epidemic and critical issues or it is mandatory to be an expert ?

Due to the pandemic of coronavirus, the most source that the people depend on was social media. There is no guaranteed solution that is biased from political opinion. An example of that the...

01 February 2021 7,567 3 View

In your opinion, Which are conditions more dangerous for the peoples of the world?

In your opinion, Which are conditions more dangerous for the peoples of the world? Ignorance of peoples, Poverty of peoples, Enslavement of peoples, Epidemics spread. Share us your opinion, and...

21 January 2021 5,974 80 View

Is the globalization concept under threat by the effect of Covid-19?

Is the globalization concept under threat by the effect of Covid-19? Because Nobody knows how long the COVID-19 epidemic will last. People around the world may have to wait until a vaccine is...

02 January 2021 8,155 1 View

What is the new in new year 2021?

2020is finished and was very hard for millions now how to welcome new year.now what is our wishes in new year e year 2020 has been an unpredictable ride through the pandemic, protests and many...

01 January 2021 1,200 5 View

Why most of the student and teacher of the university wanting to retune to university campuses ?

with knowing that they might be at risk of the epidemic. The rapid evolution of online learning does not affect that desire to return to traditional learning. is the reason of that socially, or...

29 December 2020 899 9 View

Is the quality of the article published in Renowned Journal (Elsevier, Wiley etc.)and open access renowned journals (MDPI,Frontiers etc.)are the same?

It is well known that the quality of the article published in renowned journals like Elsevier, Wiley, Springer, Tailor& Francis etc. are very good. Besides, several renowned open access...

28 December 2020 6,256 58 View

Do you trust the COVID 19 vaccines launched on the market? Do you think they will be able to stop this epidemic?

Do you trust the COVID 19 vaccines launched on the market? Do you think they will be able to stop this epidemic?

26 December 2020 9,015 97 View

What are the negative aspects of the COVID-19 epidemic on the well-being of young people who are unemployed?

What has been your experience in your respective country?

22 December 2020 663 3 View

What role did digitalisation play in the oil and gas industry in the COVID-19 epidemic?

As known, the covid epidemic has made big changes in the work of entire sectors of the economy. In Russia, not so long ago, the process of digitalization in the oil and gas industry began, which...

16 December 2020 6,499 2 View