Hi everyone,
I was just wondering if people would be willing to share their tips and tricks with regards to generating the cleanest possible DNA electrophoresis gels.
Some of the ones I've emplyed include: using freshly made running buffer, making sure the buffer doesn't get too hot, and loading just the right amount of DNA. I think this is the sort of thing where many people will have personal anecdotes rather than publications they can point to, so I'd love to hear about anything that works even in just your hands or your lab's.
Essentially I'm trying to go from pretty good gels to excellent gels.
Thanks for your help!
Well sir as far as my work is concerned, I always keep few things in mind while working with the Gel for Electrophoresis purpose like
Use freshly prepared buffer coz if it's kept too long or running more samples in it the salt concentration gets disturbed and the current face some resistance which can affect the bands running from one terminal to other.
I Prefer to make 1.5% gel, care must be taken to use the comb size and wells u need to run a sample.
Loading the right amount fo DNA as u mentioned is always important. I used 2ul loading dye with 5ul DNA.
Ladder size and amount also matters.
Proper running time should be noted and lastly, the staining in Ethidium Bromide is the key step. It should be made fresh after every 4 to 5 days. Old stain cannot able to show clear bands as fresh one and old one takes more staining time as fresh
Regards
Sanaullah Sajid
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