im going to test antimicrobial activity of sargassum seaweed methanol extract. But im confused as to which concentrations should i work on and how to prepare such concentrations? also concentrations should be in micrograms or milligrams?
Hi Shruti
Not sure exactly what your aim is but surely testing the effects of different concentration of the extracts will be part of the study? A good place to start may be using the pure extract and doing a serial dilution to see at what concentration it is still effective at.
Here is a reference for paper that may be of some help.
El-baky, H. H. A., Baz, F. K. El, & El-baroty, G. S. (2008). EVALUATION OF MARINE ALGA ULVA LACTUCA L . AS A SOURCEOF NATURAL PRESERVATIVE INGREDIENT. Electronic Journal of Enviromental, Agricultural and Food Chemistry, 7(11), 3353–3367.
Regards
Mark
First you dried your extract and weigh it.
Made a stock concentration of extract like 1mg/ml or 10mg/ml.
From this you can prepare several grade of solution of your extract.
e.g. 100microgram/ml 200micro./ml, 300micro/ml and so on....
then you can use disc diffusion assay or serial dilution assay to test the antimicrobial activity from these concentrations.
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