Comparing Internals gluconate based internal solutions use for patching

01 January 1970 0 9K Report

When looking for gluconate based internal solutions for patching, I am hoping to get some help and input as to the variations in the chemicals added. I was researching into papers that are patching Purkinje cells, generation of LTD as well as filling with biocytin... I also read that cesium based internals are not suitable for biocytin labelling ...

Could I please have some input into why

Sodium versus Magnesium ATP and GTPs...

Addition of sucrose in some internals

Some groups use Sodium Chloride rather than Pot Chloride (would Pot be better?)

Thanks!!!!

Sindy

Badges
Science topic

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

I want to remove TiO2 and SiO2 coating from surface. any one can help me?

I want to remove TiO2 and SiO2 coating from surface. I think that i should use HCl and MgCl2 and an oxidation agent such as potassium nitrate, but i dont know in which propertion should these...

14 February 2021 300 3 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

How to determinate of molecular structure of salt into the liquid?

I need to determine the open formula of our complex, presumably consisting of sodium tungstate Na2WO4 and aluminum sulfate Al2 (SO4) 3. Our problem is that with an increase in the concentration...

11 February 2021 5,821 1 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

References for relative humidity created by salt solutions at different temperatures?

I understand that the ability of standard salt solutions commonly used to produce a certain relative humidity (RH) in a closed space changes somewhat with the temperature. Where do I find data...

04 February 2021 7,370 4 View