Aniruddha Das

8 Questions 4 Answers 0 Followers

Questions related from Aniruddha Das

Commercially available antibody to detect yeast proteasome subunit?

I am wondering which are the antibody commercially available to detect specifically the proteasome subunits in budding yeast (Saccharomyces cerevisiae). Any suggestions and recommendations really...

18 July 2023 5,166 0 View

How to study about a novel protein and its function, if there is no database or antibody is available to study the expression profile of that protein?

If I want to study about any novel protein (no database) and understand its role and functionality, what approaches can we follow?

10 January 2016 5,286 4 View

Where are cytoplasmic proteins localize in the cytosol and how are they regulated?

Generally, all the membrane proteins localized specific destination inside the cell by different mechanism, like organelle specific protein destined by its signal sequence, it is true for membrane...

19 February 2014 6,749 1 View

How to promote cell growth without cell division?

Generally, in the G phase of cell cycle cell growth occurs. Due to S phase, replication of DNA as well as synthesis of proteins require for cell division to occur. So, is it possible to increase...

27 January 2014 4,592 9 View

What are the differences between human embryonic stem cells (hESCs) and mouse embryonic stem cells (mESCs)?

In terms of maintenance and differentiation, the potential of hESCs and mESCs differ. What are the differences between these two cell types and what are the ways to culture these two different...

23 January 2014 4,288 1 View

How to study protein localization through Time-Lapse microscopy in live mouse Embryonic stem cell in-vitro at the single cell level?

I want to study protein localization in single cell level by Time-Lapse microscope. Please kindly suggest me the proper methodology or protocol to study the live cell. Thanks in advance for your...

19 January 2014 5,083 3 View

Does anyone have Cdc42 knockout mouse embryonic stem cell line?

I required the Cdc42 knockout mouse embryonic stem cell for my experiment. Does anyone have this cell line or can anybody suggest from where I may get this cell line. If it is possible please let...

17 January 2014 2,983 1 View

How can I synchronize the cell?

I want to study protein localization during cell division and before cytokinesis. So, for that I want to synchronize the cells. Please suggest how I can achieve this.

17 January 2014 4,170 5 View