Only CD8 positive cells in hPMBC humanized model, no CD4 cells?

We have been using hPBMC-NOG mice for humanized mice. After tumor has grown to a 800mm3, we have done immunophenotyping of these tumors. We consistently observing that CD4+ cells are completely...

03 April 2019 9,616 1 View

Cetuximab Resistant PDx models?

Looking for a CRO/academic collaboration who has cetuximab resistant HNSCC/CRC pdx models. Anybody has these models?

02 March 2018 3,158 1 View

Can anybody suggest a good metastasis model in mouse?

My intention is to show that my drug inhibits metastasis. Preferably, looking for non-tail vein injection method. May be a good cell line which metastasize to distal place when it is implanted...

01 February 2018 9,494 6 View

I am looking for a Hu-EGFR and mouse-EGFR plasmid for stable transfections. Anybody have it? Any commercial sources?

I need to stably transfect hu-EGFR and mouse-EGFR in murine cell lines, seperately. Please help us.

01 February 2018 3,679 3 View

Where to buy Luciferase Plasmid for profit organizations?

Hi, I work for a profit organization. I was trying to buy a firefly luciferase plasmid to transfect into cell lines for Caliper IVIS in vivo xenografts luminescence imaging. I know, Addgene is a...

01 February 2018 1,556 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View