Only CD8 positive cells in hPMBC humanized model, no CD4 cells?

We have been using hPBMC-NOG mice for humanized mice. After tumor has grown to a 800mm3, we have done immunophenotyping of these tumors. We consistently observing that CD4+ cells are completely...

03 April 2019 9,616 1 View

Commercial Source for Immortalized Human Killer Cells?

Hi all, I am planning for ADCC (not with PBMCs) assay and looking for a commercial source for immortalized CD3-CD56+ Natural Killer cells. I know ATCC has NK-92 cells, but I am looking for an...

03 April 2018 7,516 1 View

Can anybody suggest a good metastasis model in mouse?

My intention is to show that my drug inhibits metastasis. Preferably, looking for non-tail vein injection method. May be a good cell line which metastasize to distal place when it is implanted...

01 February 2018 9,494 6 View

I am looking for a Hu-EGFR and mouse-EGFR plasmid for stable transfections. Anybody have it? Any commercial sources?

I need to stably transfect hu-EGFR and mouse-EGFR in murine cell lines, seperately. Please help us.

01 February 2018 3,679 3 View

Where to buy Luciferase Plasmid for profit organizations?

Hi, I work for a profit organization. I was trying to buy a firefly luciferase plasmid to transfect into cell lines for Caliper IVIS in vivo xenografts luminescence imaging. I know, Addgene is a...

01 February 2018 1,556 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

Recommendations on CO2 Resistant Rocking Shaker?

A certain protocol we have developed requires the use of a Rocker/Rocking Shaker in which our dishes with cells are maintained in the incubator. The rocking is essential to the protocol but we...

09 February 2021 3,291 1 View

Which is the most accepted taxonomy in Resistance to Change literature?

I am searching for a well accepted taxonomy of resistance to change and in terms of its underlying causes/roots.

04 February 2021 497 3 View