03 March 2018 1 3K Report

Looking for a CRO/academic collaboration who has cetuximab resistant HNSCC/CRC pdx models. Anybody has these models?

More Srinivas Reddy's questions See All
Only CD8 positive cells in hPMBC humanized model, no CD4 cells?

We have been using hPBMC-NOG mice for humanized mice. After tumor has grown to a 800mm3, we have done immunophenotyping of these tumors. We consistently observing that CD4+ cells are completely...

03 April 2019 9,665 1 View

Commercial Source for Immortalized Human Killer Cells?

Hi all, I am planning for ADCC (not with PBMCs) assay and looking for a commercial source for immortalized CD3-CD56+ Natural Killer cells. I know ATCC has NK-92 cells, but I am looking for an...

03 April 2018 7,557 1 View

Can anybody suggest a good metastasis model in mouse?

My intention is to show that my drug inhibits metastasis. Preferably, looking for non-tail vein injection method. May be a good cell line which metastasize to distal place when it is implanted...

01 February 2018 9,548 6 View

I am looking for a Hu-EGFR and mouse-EGFR plasmid for stable transfections. Anybody have it? Any commercial sources?

I need to stably transfect hu-EGFR and mouse-EGFR in murine cell lines, seperately. Please help us.

01 February 2018 3,719 3 View

Where to buy Luciferase Plasmid for profit organizations?

Hi, I work for a profit organization. I was trying to buy a firefly luciferase plasmid to transfect into cell lines for Caliper IVIS in vivo xenografts luminescence imaging. I know, Addgene is a...

01 February 2018 1,587 2 View

Similar questions and discussions
What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

My rosetta strain has chloramp resistance and i am transforming my plasmid with amp resistance but not getting colonies on chloramp/amp LB plates?

I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?

12 July 2024 9,904 1 View

Quelles applications innovantes peuvent bénéficier des propriétés des géopolymère?

Les géopolymères offrent plusieurs propriétés intéressantes qui peuvent être exploitées dans diverses applications innovantes : 1. Construction durable : - Bétons à faible empreinte carbone -...

10 July 2024 7,457 1 View

What is alternative for PMMA?

If we want to introduce a material similar to polymethyl methacrylate "with the characteristics mentioned in the next paragraph" that can be added to a precursor and create a composite, which...

03 July 2024 2,371 1 View

How can we reduce the porosity of tungsten oxide (WO3) thin films during DC sputtering deposition (target: W, power: 100 W, 75 W, and 50 W)?

We are fabricating a tandem structure where a ~50 nm thick WO3 film is deposited between two metal films using DC sputtering. We use a mixture of Ar and O2 gases at different flow rates while...

01 July 2024 2,686 2 View

Hi i m workiking with hct116 resistant and naïve cells after passage half of my cells die can anybody help?

thanks

25 June 2024 4,667 0 View

Why carrier Concentration decreases while increasing Temperature ?

Hi All . Recently, I conducted Hall measurement experiments at high temperatures on highly p-doped diamond with a doping concentration of 2.5×10^20 cm−3 During these experiments, I observed two...

23 June 2024 4,763 0 View

Why carrier Concentration decreases while increasing Temperature ?

Hi All . Recently, I conducted Hall measurement experiments at high temperatures on highly p-doped diamond with a doping concentration of 2.5×10^20 cm−3 During these experiments, I observed two...

23 June 2024 6,583 2 View

Effect of post bake on PPR resist?

HI , i am concerned about any resist hardening effect of Post bake my LOR +PPR coated sample @90C for 5 mins. I wanted to do liftoff in acetone but the metals are hard to peel off. I cant use PG...

18 June 2024 5,741 0 View

Why should you convert a WGS to core genome? and what is a core genome?

I read a paper where they converted WGS to core genome to check for AMR through resfinder, any insights would be appreciated, new to bioinformatics.

10 June 2024 8,347 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close