What is the red autofluorescence in my HL60 cells?

A subset of my HL60 cells has red autofluorescence in the far red region (FL-3 > 670 nm). I first noticed this some months ago when I was checking an antibody with a PE-Cy5 tag by flow...

06 December 2020 6,005 2 View

Best ways to collect saliva from guinea pigs?

Hello, I am planning a reserch which involves analysis of saliva cortisol in guinea pigs. Unfortunately, I am currently having trouble collecting saliva samples from my guinea pigs. I am not able...

30 September 2020 9,226 1 View

Transfection in HFL-1 cells?

I am trying to transfect HFL-1 cells with a plasmid DNA. I used Lipofectamine 3000 from ThermoScientific, however the cells seem to react quite sensitive to the transfection (tried different...

17 November 2019 484 2 View

Is there a full list of scales from the FOCUS Questionnaire (van Muijen et al., 1999)?

Hi! I'm currently working on my Master's thesis and looking for the evaluative scales for the innovation and rules value orientation from the FOCUS Questionnaire developed by van Muijen et al....

05 May 2019 4,039 3 View

I have putted the 10fold amount of 2-Mercaptoethanol in my stripping buffer by mistake. Does anyone have experience with such a mistake?

I think my proteins on the PVDF membrane are gone, because there is nothing to detect anymore. But on the other hand the membranes smell after a lot of washing procedures still of 2-ME. Maybe my...

18 July 2018 171 3 View

Is there any relevance theoretical work that addresses the question of folk psychology?

This question is in connection with an article I am writing. Relevance theory seems potentially helpful for my argument, but I need to find work that explicitly addresses the link between the...

28 September 2015 8,493 3 View

Can anyone provide advice on how to model age captured in categories within SEM?

10 July 2015 4,972 15 View

How can I regenerate a Pt surface after polymerization?

I have a Pt crystal I would like to reuse. The Pt surface has been electrochemically grafted with a diazonium salt and subsequently polymerized with GMA. I'm thinking it should be possible to...

09 July 2015 5,149 2 View

Is there any recent work on literary language (or the nature thereof) or of "literariness" more generally?

Critics don't seem to be interested anymore in distinguishing between ordinary and literary language, but I'm wondering whether this is a crucial issue we need to return to.

06 October 2014 8,200 5 View

Can anyone suggest a material with the highest specific heat capacity (higher than water)?

Hi, I was wondering which material has the highest specific heat capacity? Most lists I could find have water at the top with 4.182 kJ/kgK. But I was wondering if there is a solid or liquid...

17 September 2014 5,850 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View