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I ordered two Predesigned assays for quantification of CpG methylation by Pyrosequencing from Qiagen and I do not have any product after PCR. I have dissolved them in TE ph8, tried different...

22 September 2020 3,328 6 View

What does it mean to see a band for uncloned vector and one band for vector with insert from one colony after cloning?

I used gateway cloning to clone my insert into the donor vector. After having colonies, I did a mini prep and ran it on the gel. On the gel I could see a clear band on the right length for my...

14 June 2020 9,829 3 View

What are the soil nutrients use efficiencies in Central America?

Hello everyone, I am working in Central America and I would like to create a document that could help me to calculate "simply" the nutrients disponibility in the soil of coffee fields. The...

10 June 2020 9,671 4 View

How to perform gradient qPCR with Roche Light Cycler 480, to determine the best annealing temperature of my primers??

I would like to determine the best annealing temperature for my primers, giving the lowest Cq and the best efficiency. Is there a thermal gradient feature on the Roche LC480 instrument? And if so,...

16 February 2020 9,471 3 View

Which statistical analysis should I use for 2 dependent variables that are measured with a Likert Scale?

I am studying the generational changes in the relationship between empathy and social media usage. I am using a Likert Scale to measure both empathy levels and social media usage; however, I do...

20 January 2020 9,617 4 View

Is there a way to statistically separate the top percent of performers in a pre-test condition, to then analyse their scores in a post-test measure?

Essentially in a study where there is a pre-test, then an intervention and a post-test (Identical tests), I have a question: "Do students who perform better on the skill test before the...

12 December 2019 9,096 4 View

Why are my re-probings for Western blot analysis unsuccessful?

Hello everyone, I am having problems with re-probing membranes for Western blot analysis. Almost every time I want to re-probe with either the same or a new primary antibody, I don't get any...

11 December 2019 5,066 5 View

How to dissolve and inject 4-hydroxytamoxifen to nude mouse?

What is the normal way to inject 500 ug 4-hydroxytamoxifen per mouse? Is it dissolved 4-OHT in ethanol and diluted with peanut oil at least 10 times?

10 May 2019 3,301 1 View

Why does this strange situation occur in my nude mice xenograft after drug injection?

I injected cancer cells to mice as xenografts. After 7 days, I injected tamoxifen together with an inhibitor of interleukin to nude mice subcutaneously. But 2 days later, the tumor site turned to...

21 March 2019 6,082 4 View

Does anyone have experience in cleaving Fibronectin using an MMP before Western Blot analysis?

We are trying to demonstrate the our treatment effects increases the activity of MMP-2 by showing mutiple cleaved fibronectin bands in a western blot? For this we are going to have a control of...

13 February 2019 7,734 3 View

What are some alternatives to the Self-Directed Learning Readiness Scale (SDLRS) developed by Dr. Lucy L. Guglielmino?

The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...

15 February 2021 3,095 4 View

How to calculate response rate if the number of eligible respondents is not available?

Hi All, I am carrying out an international research with clinicians (speech-language pathologists/ therapists). International professional associations will be distributing the questionnaire link...

02 February 2021 5,306 3 View

How is it possible to rank variables using Likert scale?

Hello Everyone, I have a questionnaire of a 3-point Likert scale for an overall evaluation for a service, and several detailed attributes evaluation of that service. For example: Overall...

31 January 2021 9,612 8 View

How do you multiple impute item scores and perform a linear regression on factor scores?

I am developing a questionnaire and first performing an exploratory factor analysis. After I have the final factor structure, I plan on regressing the factor scores on some demographic covariates....

18 January 2021 883 8 View

How did teaching writing gain its ground in the course of time ?

Since the decline of audiolingualism, there was bias towards speech in language teaching for communicative ends. Though, the stress on speaking rather than writing produces fluent but inaccurate...

12 January 2021 517 4 View

Can you recommend good Practice methods for teaching qualatative thematic analysis in grad level qualatative research course ?

While I've been teaching this for years, I find it to be the most challenging and for students stressful aspect of becoming qualatative researchers. Have you devised any good practice pedagogies?

03 January 2021 3,292 4 View

You can mention a contribution that science will achieve in this year 2021?

It is known that scientific knowledge is replicated each time at more unusual speeds, however, humanity expects more from our scientific work, that is, solutions to problems that have accumulated...

02 January 2021 8,300 16 View

How will COVID-19 change higher education in the short/long run?

31 December 2020 5,414 68 View

How do I access Domestic Violence Attitude Scales or find them for free?

Hi, I'm a postgraduate student currently starting up a research project on attitudes towards domestic violence (DV). The study will aim to compare cultural differences between attitudes and...

31 December 2020 781 3 View

What is the best Quality of Life questionnaire for chronic patients?

Hello! I am looking for a SHORT questionnaire/scale to measure Quality of Life in chronic patients (e.g. fibromyalgia, or multiple sclerosis). Ideally, validated for English and Spanish speakers....

22 December 2020 9,962 4 View